Journal Article

PUBDB-2021-05636

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples

Schmutzler, O.CFEL*Extern* ; Graf, S. ; Behm, N. ; Mansour, W. Y. ; Blumendorf, F.CFEL*Extern* ; Staufer, T.Extern* ; Körnig, C.Extern* ; Salah, D. ; Kang, Y.Extern* ; Peters, J. N.Extern* ; Liu, Y. ; Feliu, N.Extern* ; Parak, W. J.Extern* ; Burkhardt, A.XFEL.EU*DESY* ; Gargioni, E.Extern* ; Gennis, S. ; Chandralingam, S. ; Höeg, F. ; Maison, W. ; Rothkamm, K. (Corresponding author)Extern* ; Schulz, F. (Corresponding author)Extern* ; Grüner, F. (Corresponding author)CFEL*Extern*

2021
Molecular Diversity Preservation International Basel

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Please use a persistent id in citations: doi:10.3390/ijms22073691  doi:10.3204/PUBDB-2021-05636

Abstract: Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.

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Contributing Institute(s):

1. DOOR-User (DOOR ; HAS-User)
2. Uni Hamburg / Experimentalphysik (UNI/EXP)
3. Innovation & Technologietransfer (ITT)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
3. DFG project 194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)
4. DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)

Experiment(s):

1. PETRA Beamline P11 (PETRA III)

Appears in the scientific report 2021

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Database coverage:
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