TY  - JOUR
AU  - Schmutzler, Oliver
AU  - Graf, Sebastian
AU  - Behm, Nils
AU  - Mansour, Wael Y.
AU  - Blumendorf, Florian
AU  - Staufer, Theresa
AU  - Körnig, Christian
AU  - Salah, Dina
AU  - Kang, Yanan
AU  - Peters, Jan N.
AU  - Liu, Yang
AU  - Feliu, Neus
AU  - Parak, Wolfgang J.
AU  - Burkhardt, Anja
AU  - Gargioni, Elisabetta
AU  - Gennis, Sabrina
AU  - Chandralingam, Sharah
AU  - Höeg, Finn
AU  - Maison, Wolfgang
AU  - Rothkamm, Kai
AU  - Schulz, Florian
AU  - Grüner, Florian
TI  - X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples
JO  - International journal of molecular sciences
VL  - 22
IS  - 7
SN  - 1422-0067
CY  - Basel
PB  - Molecular Diversity Preservation International
M1  - PUBDB-2021-05636
SP  - 3691
PY  - 2021
AB  - Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.
LB  - PUB:(DE-HGF)16
C6  - pmid:33916283
UR  - <Go to ISI:>//WOS:000638620000001
DO  - DOI:10.3390/ijms22073691
UR  - https://bib-pubdb1.desy.de/record/473306
ER  -