Publications database

In the contemporary context of research, financial expenditures are escalating at an alarmingrate.This encompasses not only direct expenses associated with the research process, such asstaff, hardware, and equipment, but also the dissemination and archiving of results. Usually,these costs remain inaccessible to the public, a state of affairs that is deemed to be highlyunsatisfactory. [...]

Split inteins catalyze protein trans-splicing by ligating their extein sequences while undergoing self-excision, enabling diverse protein modification applications. However, many purified split intein precursors exhibit partial or no splicing activity for unknown reasons. [...]

A measurement of partial branching fractions of charmless semileptonic beauty meson decaysusing an inclusively reconstructed hadronic system is presented. These decays are denoted asB → Xuℓν, where Xu represents a hadronic system containing a u quark produced in the un-derlying quark-level b → u transition. [...]

Using high-pressure single-crystal x-ray diffraction combined with thermodynamic measurements and density-functional calculations, we uncover the microscopic magnetic model of the mineral brochantite, Cu$_4$⁢SO$_4$⁢(OH)$_6$, and its evolution upon compression. The formation of antiferromagnetic spin chains with the effective intrachain coupling of 𝐽≃100K is attributed to the occurrence of longer Cu–Cu distances and larger Cu–O–Cu bond angles between the structural chains within the layers of the brochantite structure [...]

Ecto-5’-nucleotidase (CD73) is the main enzyme that catalyzes the hydrolysis of extracellular AMP to produce anti-inflammatory, immunosuppressive adenosine. Many tumor cells over-express ectonucleotidases accumulating adenosine in the tumor microenvironment, which promotes tumor growth, metastasis, angiogenesis, and immune escape. [...]

Upregulation of the actin-severing protein Cofilin-1 is implicated in enhancing malignancy of various cancer types by promoting actin turnover and increasing cellular motility. Despite the importance of targeting Cofilin-1, currently there is a lack of inhibitors specifically targeting its actin-severing activity. [...]

Pseudomonas aeruginosa is a prevalent opportunistic human pathogen, particularly associated with cystic fibrosis. Among its virulence factors are the LecA and LecB lectins. [...]

While protein A affinity chromatography is widely established for antibody purification, the acidic elution conditions often lead to protein aggregation and deamidation. Here, an alternative approach is described for the purification of antibodies utilizing an engineered binding protein based on the archaebacterial Sac7d scaffold in combination with light-controlled α-CD affinity chromatography (Excitography). [...]

Nonribosomal peptide synthetases (NRPSs) are multi-domain enzymes that catalyze the biosynthesis of therapeutically relevant natural products. Efficient peptide synthesis relies on intricate domain interactions, whose underlying principles remain poorly understood. [...]

Helical aromatic oligoamide foldamers bearing anionic side chains that mimic the overall shape and charge surface distribution of DNA were synthesized. Their interactions with chromosomal protein Sac7d, a non-sequence-selective DNA-binder that kinks DNA, were investigated by Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Circular Dichroism spectroscopy (CD), melting curve analysis, Atomic Force Microscopy (AFM), and Nuclear Magnetic Resonance (NMR), as well as by single crystal X-ray crystallography. [...]