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000473306 1001_ $$0P:(DE-H253)PIP1027798$$aSchmutzler, Oliver$$b0
000473306 245__ $$aX-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples
000473306 260__ $$aBasel$$bMolecular Diversity Preservation International$$c2021
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000473306 520__ $$aQuantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.
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000473306 7001_ $$0P:(DE-HGF)0$$aGraf, Sebastian$$b1
000473306 7001_ $$0P:(DE-HGF)0$$aBehm, Nils$$b2
000473306 7001_ $$00000-0003-0641-2885$$aMansour, Wael Y.$$b3
000473306 7001_ $$0P:(DE-H253)PIP1017935$$aBlumendorf, Florian$$b4
000473306 7001_ $$0P:(DE-H253)PIP1031573$$aStaufer, Theresa$$b5
000473306 7001_ $$0P:(DE-H253)PIP1083195$$aKörnig, Christian$$b6
000473306 7001_ $$0P:(DE-HGF)0$$aSalah, Dina$$b7
000473306 7001_ $$0P:(DE-H253)PIP1094950$$aKang, Yanan$$b8
000473306 7001_ $$0P:(DE-H253)PIP1030854$$aPeters, Jan N.$$b9
000473306 7001_ $$0P:(DE-HGF)0$$aLiu, Yang$$b10
000473306 7001_ $$0P:(DE-H253)PIP1085824$$aFeliu, Neus$$b11
000473306 7001_ $$0P:(DE-H253)PIP1085825$$aParak, Wolfgang J.$$b12
000473306 7001_ $$0P:(DE-H253)PIP1010228$$aBurkhardt, Anja$$b13
000473306 7001_ $$0P:(DE-H253)PIP1092156$$aGargioni, Elisabetta$$b14
000473306 7001_ $$0P:(DE-HGF)0$$aGennis, Sabrina$$b15
000473306 7001_ $$0P:(DE-HGF)0$$aChandralingam, Sharah$$b16
000473306 7001_ $$00000-0002-8891-5250$$aHöeg, Finn$$b17
000473306 7001_ $$0P:(DE-HGF)0$$aMaison, Wolfgang$$b18
000473306 7001_ $$0P:(DE-H253)PIP1033154$$aRothkamm, Kai$$b19$$eCorresponding author
000473306 7001_ $$0P:(DE-H253)PIP1019230$$aSchulz, Florian$$b20$$eCorresponding author
000473306 7001_ $$0P:(DE-H253)PIP1013695$$aGrüner, Florian$$b21$$eCorresponding author
000473306 773__ $$0PERI:(DE-600)2019364-6$$a10.3390/ijms22073691$$gVol. 22, no. 7, p. 3691 -$$n7$$p3691$$tInternational journal of molecular sciences$$v22$$x1422-0067$$y2021
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