%0 Journal Article
%A Schmutzler, Oliver
%A Graf, Sebastian
%A Behm, Nils
%A Mansour, Wael Y.
%A Blumendorf, Florian
%A Staufer, Theresa
%A Körnig, Christian
%A Salah, Dina
%A Kang, Yanan
%A Peters, Jan N.
%A Liu, Yang
%A Feliu, Neus
%A Parak, Wolfgang J.
%A Burkhardt, Anja
%A Gargioni, Elisabetta
%A Gennis, Sabrina
%A Chandralingam, Sharah
%A Höeg, Finn
%A Maison, Wolfgang
%A Rothkamm, Kai
%A Schulz, Florian
%A Grüner, Florian
%T X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples
%J International journal of molecular sciences
%V 22
%N 7
%@ 1422-0067
%C Basel
%I Molecular Diversity Preservation International
%M PUBDB-2021-05636
%P 3691
%D 2021
%X Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:33916283
%U <Go to ISI:>//WOS:000638620000001
%R 10.3390/ijms22073691
%U https://bib-pubdb1.desy.de/record/473306