Journal Article

PUBDB-2024-05664

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The palisade layer of the poxvirus core is composed of flexible A10 trimers

Liu, J. ; Corroyer-Dulmont, S.Extern*CSSB* ; Pražák, V. ; Khusainov, I. ; Bahrami, K. ; Welsch, S. ; Vasishtan, D.Extern*CSSB* ; Obarska-Kosińska, A. ; Thorkelsson, S. R.Extern*CSSB* ; Gruenewald, K. (Corresponding author)CSSB* ; Quemin, E. (Corresponding author)Extern*CSSB* ; Turoňová, B. (Corresponding author) ; Locker, J. K. (Corresponding author)

2024
Nature Publishing Group London [u.a.]

This record in other databases:        

Please use a persistent id in citations: doi:10.1038/s41594-024-01218-5  doi:10.3204/PUBDB-2024-05664

Abstract: Due to its asymmetric shape, size and compactness, the structure of the infectious mature virus (MV) of vaccinia virus (VACV), the best-studied poxvirus, remains poorly understood. Instead, subviral particles, in particular membrane-free viral cores, have been studied with cryo-electron microscopy. Here, we compared viral cores obtained by detergent stripping of MVs with cores in the cellular cytoplasm, early in infection. We focused on the prominent palisade layer on the core surface, combining cryo-electron tomography, subtomogram averaging and AlphaFold2 structure prediction. We showed that the palisade is composed of densely packed trimers of the major core protein A10. Trimers display a random order and their classification indicates structural flexibility. A10 on cytoplasmic cores is organized in a similar manner, indicating that the structures obtained in vitro are physiologically relevant. We discuss our results in the context of the VACV replicative cycle, and the assembly and disassembly of the infectious MV.

---

Contributing Institute(s):

1. CSSB - Leibniz-Institut für Experimentelle Virologie (LIV) - Kay Grünewald (CSSB-LIV-KG)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2024

---

Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Nature ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 15 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---