TY  - JOUR
AU  - Liu, Jiasui
AU  - Corroyer-Dulmont, Simon
AU  - Pražák, Vojtěch
AU  - Khusainov, Iskander
AU  - Bahrami, Karola
AU  - Welsch, Sonja
AU  - Vasishtan, Daven
AU  - Obarska-Kosińska, Agnieszka
AU  - Thorkelsson, Sigurdur R.
AU  - Gruenewald, Kay
AU  - Quemin, Emmanuelle
AU  - Turoňová, Beata
AU  - Locker, Jacomina Krijnse
TI  - The palisade layer of the poxvirus core is composed of flexible A10 trimers
JO  - Nature structural & molecular biology
VL  - 31
IS  - 7
SN  - 1545-9993
CY  - London [u.a.]
PB  - Nature Publishing Group
M1  - PUBDB-2024-05664
SP  - 1105-1113
PY  - 2024
AB  - Due to its asymmetric shape, size and compactness, the structure of the infectious mature virus (MV) of vaccinia virus (VACV), the best-studied poxvirus, remains poorly understood. Instead, subviral particles, in particular membrane-free viral cores, have been studied with cryo-electron microscopy. Here, we compared viral cores obtained by detergent stripping of MVs with cores in the cellular cytoplasm, early in infection. We focused on the prominent palisade layer on the core surface, combining cryo-electron tomography, subtomogram averaging and AlphaFold2 structure prediction. We showed that the palisade is composed of densely packed trimers of the major core protein A10. Trimers display a random order and their classification indicates structural flexibility. A10 on cytoplasmic cores is organized in a similar manner, indicating that the structures obtained in vitro are physiologically relevant. We discuss our results in the context of the VACV replicative cycle, and the assembly and disassembly of the infectious MV. 
LB  - PUB:(DE-HGF)16
C6  - pmid:38316878
UR  - <Go to ISI:>//WOS:001158144600003
DO  - DOI:10.1038/s41594-024-01218-5
UR  - https://bib-pubdb1.desy.de/record/613885
ER  -