Journal Article

PUBDB-2024-05621

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Molecular plasticity of herpesvirus nuclear egress analysed in situ

Prazak, V.CSSB* ; Mironova, Y.CSSB* ; Vasishtan, D.CSSB* ; Hagen, C.CSSB* ; Laugks, U.CSSB* ; Jensen, Y.CSSB* ; Sanders, S.CSSB* ; Heumann, J. M. ; Bosse, J. B.CSSB* ; Klupp, B. G. ; Mettenleiter, T. C. ; Grange, M. (Corresponding author) ; Gruenewald, K. (Corresponding author)CSSB*

2024
Nature Publishing Group London

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Please use a persistent id in citations: doi:10.1038/s41564-024-01716-8

Abstract: The viral nuclear egress complex (NEC) allows herpesvirus capsids to escape from the nucleus without compromising the nuclear envelope integrity. The NEC lattice assembles on the inner nuclear membrane and mediates the budding of nascent nucleocapsids into the perinuclear space and their subsequent release into the cytosol. Its essential role makes it a potent antiviral target, necessitating structural information in the context of a cellular infection. Here we determined structures of NEC–capsid interfaces in situ using electron cryo-tomography, showing a substantial structural heterogeneity. In addition, while the capsid is associated with budding initiation, it is not required for curvature formation. By determining the NEC structure in several conformations, we show that curvature arises from an asymmetric assembly of disordered and hexagonally ordered lattice domains independent of pUL25 or other viral capsid vertex components. Our results advance our understanding of the mechanism of nuclear egress in the context of a living cell.

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Contributing Institute(s):

1. CSSB - Leibniz-Institut für Experimentelle Virologie (LIV) - Kay Grünewald (CSSB-LIV-KG)
2. CSSB-MHH-JB (CSSB-MHH-JB)
3. CSSB-CF-CRYO (CSSB-CF-CRYO)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)
2. DFG project G:(GEPRIS)390874280 - EXC 2155: RESIST - Resolving Infection Susceptibility (390874280) (390874280)
3. GRK 2771 - GRK 2771: Mensch und Mikrobe: Reorganisation von Zellkompartimenten und Molekülkomplexen während der Infektion (453548970) (453548970)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2024

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DEAL Nature ; Essential Science Indicators ; IF >= 25 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

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