TY  - JOUR
AU  - Prazak, Vojtech
AU  - Mironova, Yuliia
AU  - Vasishtan, Daven
AU  - Hagen, Christoph
AU  - Laugks, Ulrike
AU  - Jensen, Yannick
AU  - Sanders, Saskia
AU  - Heumann, John M.
AU  - Bosse, Jens Bernhard
AU  - Klupp, Barbara G.
AU  - Mettenleiter, Thomas C.
AU  - Grange, Michael
AU  - Gruenewald, Kay
TI  - Molecular plasticity of herpesvirus nuclear egress analysed in situ
JO  - Nature microbiology
VL  - 9
IS  - 7
SN  - 2058-5276
CY  - London
PB  - Nature Publishing Group
M1  - PUBDB-2024-05621
SP  - 1842-1855
PY  - 2024
N1  - Waiting for fulltext
AB  - The viral nuclear egress complex (NEC) allows herpesvirus capsids to escape from the nucleus without compromising the nuclear envelope integrity. The NEC lattice assembles on the inner nuclear membrane and mediates the budding of nascent nucleocapsids into the perinuclear space and their subsequent release into the cytosol. Its essential role makes it a potent antiviral target, necessitating structural information in the context of a cellular infection. Here we determined structures of NEC–capsid interfaces in situ using electron cryo-tomography, showing a substantial structural heterogeneity. In addition, while the capsid is associated with budding initiation, it is not required for curvature formation. By determining the NEC structure in several conformations, we show that curvature arises from an asymmetric assembly of disordered and hexagonally ordered lattice domains independent of pUL25 or other viral capsid vertex components. Our results advance our understanding of the mechanism of nuclear egress in the context of a living cell.
LB  - PUB:(DE-HGF)16
C6  - pmid:38918469
UR  - <Go to ISI:>//WOS:001254317500003
DO  - DOI:10.1038/s41564-024-01716-8
UR  - https://bib-pubdb1.desy.de/record/613733
ER  -