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@ARTICLE{Prazak:613733,
author = {Prazak, Vojtech and Mironova, Yuliia and Vasishtan, Daven
and Hagen, Christoph and Laugks, Ulrike and Jensen, Yannick
and Sanders, Saskia and Heumann, John M. and Bosse, Jens
Bernhard and Klupp, Barbara G. and Mettenleiter, Thomas C.
and Grange, Michael and Gruenewald, Kay},
title = {{M}olecular plasticity of herpesvirus nuclear egress
analysed in situ},
journal = {Nature microbiology},
volume = {9},
number = {7},
issn = {2058-5276},
address = {London},
publisher = {Nature Publishing Group},
reportid = {PUBDB-2024-05621},
pages = {1842-1855},
year = {2024},
note = {Waiting for fulltext},
abstract = {The viral nuclear egress complex (NEC) allows herpesvirus
capsids to escape from the nucleus without compromising the
nuclear envelope integrity. The NEC lattice assembles on the
inner nuclear membrane and mediates the budding of nascent
nucleocapsids into the perinuclear space and their
subsequent release into the cytosol. Its essential role
makes it a potent antiviral target, necessitating structural
information in the context of a cellular infection. Here we
determined structures of NEC–capsid interfaces in situ
using electron cryo-tomography, showing a substantial
structural heterogeneity. In addition, while the capsid is
associated with budding initiation, it is not required for
curvature formation. By determining the NEC structure in
several conformations, we show that curvature arises from an
asymmetric assembly of disordered and hexagonally ordered
lattice domains independent of pUL25 or other viral capsid
vertex components. Our results advance our understanding of
the mechanism of nuclear egress in the context of a living
cell.},
cin = {CSSB-LIV-KG / CSSB-MHH-JB / CSSB-CF-CRYO},
ddc = {570},
cid = {I:(DE-H253)CSSB-LIV-KG-20220525 /
I:(DE-H253)CSSB-MHH-JB-20210520 /
I:(DE-H253)CSSB-CF-CRYO-20210520},
pnm = {899 - ohne Topic (POF4-899) / DFG project
G:(GEPRIS)390874280 - EXC 2155: RESIST - Resolving Infection
Susceptibility (390874280) / GRK 2771 - GRK 2771: Mensch und
Mikrobe: Reorganisation von Zellkompartimenten und
Molekülkomplexen während der Infektion (453548970)},
pid = {G:(DE-HGF)POF4-899 / G:(GEPRIS)390874280 /
G:(GEPRIS)453548970},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38918469},
UT = {WOS:001254317500003},
doi = {10.1038/s41564-024-01716-8},
url = {https://bib-pubdb1.desy.de/record/613733},
}