Journal Article

PUBDB-2023-05545

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Commissioning the digital mass-filter/ion-trap module for the MS SPIDOC prototype

Simke, F. (Corresponding author)Extern* ; Fischer, P.Extern* ; Damjanović, T.CSSB*XFEL.EU* ; Kádek, A.CSSB*XFEL.EU* ; Kierspel, T.CSSB*DESY* ; Lorenzen, K.XFEL.EU* ; Uetrecht, C.CSSB* ; Schweikhard, L.Extern*

2023
Inst. of Physics London

This record in other databases:    

Please use a persistent id in citations: doi:10.1088/1748-0221/18/10/P10021  doi:10.3204/PUBDB-2023-05545

Abstract: The combination of a linear quadrupole ion-filter and linear Paul trap operated with a rectangular guiding field for the filtering and accumulation of ions within the Mass Spectrometry for Single Particle Imaging of Dipole Oriented protein Complexes (MS SPIDOC) prototype [T. Kierspel et al., Anal. Bioanal. Chem., published online] is characterized. Using cationic caesium-iodide clusters, the ion-separation performance, ion accumulation, cooling, and ejection via in-trap pin electrodes is evaluated. Furthermore, proof-of-principle measurements are performed with 64 kDa multiply-charged non-covalent protein complexes of human hemoglobin and 804 kDa non-covalent complex of GroEL, to demonstrate that the module meets the criteria to handle high-mass ions which are the main objective of the MS SPIDOC project. The setup's performance is found to be in line with previous results from ion-trajectory simulations [F. Simke et al., Int. J. Mass Spectrom.473 (2022) 116779].

---

Contributing Institute(s):

1. CSSB - Leibniz-Institut für Experimentelle Virologie (LIV) / DESY - Charlotte Uetrecht (CSSB-LIV/DESY-CU)
2. Sample Environment and Characterisation (XFEL_E2_SEC)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. MS SPIDOC - Mass Spectrometry for Single Particle Imaging of Dipole Oriented protein Complexes (801406) (801406)

Experiment(s):

1. Experiments at XFEL

Appears in the scientific report 2023

---

Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; National-Konsortium ; SCOPUS ; Web of Science Core Collection

---