TY  - JOUR
AU  - Simke, Florian
AU  - Fischer, Paul
AU  - Damjanović, Tomislav
AU  - Kádek, Alan
AU  - Kierspel, Thomas
AU  - Lorenzen, Kristina
AU  - Uetrecht, Charlotte
AU  - Schweikhard, Lutz
TI  - Commissioning the digital mass-filter/ion-trap module for the MS SPIDOC prototype
JO  - Journal of Instrumentation
VL  - 18
IS  - 10
SN  - 1748-0221
CY  - London
PB  - Inst. of Physics
M1  - PUBDB-2023-05545
SP  - P10021 
PY  - 2023
AB  - The combination of a linear quadrupole ion-filter and linear Paul trap operated with a rectangular guiding field for the filtering and accumulation of ions within the Mass  Spectrometry for Single Particle Imaging of Dipole  Oriented protein Complexes (MS SPIDOC) prototype [T. Kierspel et al.,  Anal. Bioanal. Chem., published online] is characterized. Using cationic caesium-iodide clusters, the ion-separation performance, ion accumulation, cooling, and ejection via in-trap pin electrodes is evaluated. Furthermore, proof-of-principle measurements are performed with 64 kDa multiply-charged non-covalent protein complexes of human hemoglobin and 804 kDa non-covalent complex of GroEL, to demonstrate that the module meets the criteria to handle high-mass ions which are the main objective of the MS SPIDOC project. The setup's performance is found to be in line with previous results from ion-trajectory simulations [F. Simke et al., Int. J. Mass Spectrom.473 (2022) 116779].  
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:001117781700010
DO  - DOI:10.1088/1748-0221/18/10/P10021
UR  - https://bib-pubdb1.desy.de/record/593879
ER  -