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100 1 _ |a Simke, Florian
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245 _ _ |a Commissioning the digital mass-filter/ion-trap module for the MS SPIDOC prototype
260 _ _ |a London
|c 2023
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520 _ _ |a The combination of a linear quadrupole ion-filter and linear Paul trap operated with a rectangular guiding field for the filtering and accumulation of ions within the Mass Spectrometry for Single Particle Imaging of Dipole Oriented protein Complexes (MS SPIDOC) prototype [T. Kierspel et al., Anal. Bioanal. Chem., published online] is characterized. Using cationic caesium-iodide clusters, the ion-separation performance, ion accumulation, cooling, and ejection via in-trap pin electrodes is evaluated. Furthermore, proof-of-principle measurements are performed with 64 kDa multiply-charged non-covalent protein complexes of human hemoglobin and 804 kDa non-covalent complex of GroEL, to demonstrate that the module meets the criteria to handle high-mass ions which are the main objective of the MS SPIDOC project. The setup's performance is found to be in line with previous results from ion-trajectory simulations [F. Simke et al., Int. J. Mass Spectrom.473 (2022) 116779].
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536 _ _ |a MS SPIDOC - Mass Spectrometry for Single Particle Imaging of Dipole Oriented protein Complexes (801406)
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700 1 _ |a Fischer, Paul
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700 1 _ |a Damjanović, Tomislav
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700 1 _ |a Kádek, Alan
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700 1 _ |a Kierspel, Thomas
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700 1 _ |a Lorenzen, Kristina
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700 1 _ |a Uetrecht, Charlotte
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700 1 _ |a Schweikhard, Lutz
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773 _ _ |a 10.1088/1748-0221/18/10/P10021
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856 4 _ |u https://iopscience.iop.org/article/10.1088/1748-0221/18/10/P10021
856 4 _ |u https://bib-pubdb1.desy.de/record/593879/files/Simke_2023_J._Inst._18_P10021.pdf
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