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000593879 1001_ $$0P:(DE-H253)PIP1097174$$aSimke, Florian$$b0$$eCorresponding author
000593879 245__ $$aCommissioning the digital mass-filter/ion-trap module for the MS SPIDOC prototype
000593879 260__ $$aLondon$$bInst. of Physics$$c2023
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000593879 520__ $$aThe combination of a linear quadrupole ion-filter and linear Paul trap operated with a rectangular guiding field for the filtering and accumulation of ions within the Mass  Spectrometry for Single Particle Imaging of Dipole  Oriented protein Complexes (MS SPIDOC) prototype [T. Kierspel et al.,  Anal. Bioanal. Chem., published online] is characterized. Using cationic caesium-iodide clusters, the ion-separation performance, ion accumulation, cooling, and ejection via in-trap pin electrodes is evaluated. Furthermore, proof-of-principle measurements are performed with 64 kDa multiply-charged non-covalent protein complexes of human hemoglobin and 804 kDa non-covalent complex of GroEL, to demonstrate that the module meets the criteria to handle high-mass ions which are the main objective of the MS SPIDOC project. The setup's performance is found to be in line with previous results from ion-trajectory simulations [F. Simke et al., Int. J. Mass Spectrom.473 (2022) 116779].  
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000593879 536__ $$0G:(EU-Grant)801406$$aMS SPIDOC - Mass Spectrometry for Single Particle Imaging of Dipole Oriented protein Complexes (801406)$$c801406$$fH2020-FETOPEN-1-2016-2017$$x1
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000593879 7001_ $$0P:(DE-H253)PIP1103382$$aFischer, Paul$$b1
000593879 7001_ $$0P:(DE-H253)PIP1101212$$aDamjanović, Tomislav$$b2
000593879 7001_ $$0P:(DE-H253)PIP1032252$$aKádek, Alan$$b3
000593879 7001_ $$0P:(DE-H253)PIP1018289$$aKierspel, Thomas$$b4
000593879 7001_ $$0P:(DE-H253)PIP1022131$$aLorenzen, Kristina$$b5
000593879 7001_ $$0P:(DE-H253)PIP1014042$$aUetrecht, Charlotte$$b6
000593879 7001_ $$0P:(DE-H253)PIP1099549$$aSchweikhard, Lutz$$b7
000593879 773__ $$0PERI:(DE-600)2235672-1$$a10.1088/1748-0221/18/10/P10021$$gVol. 18, no. 10, p. P10021 -$$n10$$pP10021 $$tJournal of Instrumentation$$v18$$x1748-0221$$y2023
000593879 8564_ $$uhttps://iopscience.iop.org/article/10.1088/1748-0221/18/10/P10021
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