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Journal Article PUBDB-2019-04293
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Time-resolved serial femtosecond crystallography at the European XFEL

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2019
Nature Publishing Group London [u.a.]

Nature methods 2020(17), 73–78 () [10.1038/s41592-019-0628-z]
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Abstract: The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition rates. This X-ray source has been designed to enable the observation of ultrafast processes with near-atomic spatial resolution. Time-resolved crystallographic investigations on biological macromolecules belong to an important class of experiments that explore fundamental and functional structural displacements in these molecules. Due to the unusual MHz X-ray pulse structure at the EuXFEL, these experiments are challenging. Here, we demonstrate how a biological reaction can be followed on ultrafast timescales at the EuXFEL. We investigate the picosecond time range in the photocycle of photoactive yellow protein (PYP) with MHz X-ray pulse rates. We show that difference electron density maps of excellent quality can be obtained. The results connect the previously explored femtosecond PYP dynamics to timescales accessible at synchrotrons. This opens the door to a wide range of time-resolved studies at the EuXFEL.

Classification:

Note: © Springer Nature Limited
Contributing Institute(s):
  1. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
  2. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
  3. FS-ML (CFEL-XOM)
Research Program(s):
  1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
  2. Leibniz Preis - Leibiz Programm 2015: Prof. Dr. Henry N. Chapman (DFG-Leibniz-2015-Chapman) (DFG-Leibniz-2015-Chapman)
  3. X-probe - Advanced XFEL and Synchrotron based Probes of Protein Structure and Dynamics (637295) (637295)
  4. AXSIS - Frontiers in Attosecond X-ray Science: Imaging and Spectroscopy (609920) (609920)
Experiment(s):
  1. Experiments at XFEL

Appears in the scientific report 2019
Database coverage:
Medline ; Embargoed OpenAccess ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 25 ; JCR ; NCBI Molecular Biology Database ; NationallizenzNationallizenz ; No Authors Fulltext ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection
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Private Collections > >CFEL > >FS-CFEL > CFEL-XOM
Private Collections > >CFEL > >FS-CFEL > CFEL-I
Private Collections > >DESY > >FS > FS-CFEL-1
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Direct Observation of the Mechanism of Antibiotic Resistance by Mix-and-Inject at the European XFEL
biorXiv () [10.1101/2020.11.24.396689]  GO OpenAccess  Download fulltext Files BibTeX | EndNote: XML, Text | RIS

 Record created 2019-11-18, last modified 2025-07-16
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Published on 2019-11-18. Available in OpenAccess from 2020-11-18.:
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