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000427972 1001_ $$0P:(DE-H253)PIP1083581$$aPandey, Suraj$$b0
000427972 245__ $$aTime-resolved serial femtosecond crystallography at the European XFEL
000427972 260__ $$aLondon [u.a.]$$bNature Publishing Group$$c2019
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000427972 520__ $$aThe European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition rates. This X-ray source has been designed to enable the observation of ultrafast processes with near-atomic spatial resolution. Time-resolved crystallographic investigations on biological macromolecules belong to an important class of experiments that explore fundamental and functional structural displacements in these molecules. Due to the unusual MHz X-ray pulse structure at the EuXFEL, these experiments are challenging. Here, we demonstrate how a biological reaction can be followed on ultrafast timescales at the EuXFEL. We investigate the picosecond time range in the photocycle of photoactive yellow protein (PYP) with MHz X-ray pulse rates. We show that difference electron density maps of excellent quality can be obtained. The results connect the previously explored femtosecond PYP dynamics to timescales accessible at synchrotrons. This opens the door to a wide range of time-resolved studies at the EuXFEL.
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000427972 7001_ $$0P:(DE-H253)PIP1020246$$aTolstikova, Alexandra$$b41
000427972 7001_ $$0P:(DE-H253)PIP1006324$$aChapman, Henry N.$$b42
000427972 7001_ $$00000-0001-7709-8331$$aRos, Alexandra$$b43
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000427972 7001_ $$0P:(DE-H253)PIP1023170$$aFromme, Petra$$b45
000427972 7001_ $$0P:(DE-H253)PIP1006340$$aMancuso, Adrian P.$$b46
000427972 7001_ $$00000-0002-0962-9468$$aSchmidt, Marius$$b47$$eCorresponding author
000427972 773__ $$0PERI:(DE-600)2163081-1$$a10.1038/s41592-019-0628-z$$n17$$p73–78$$tNature methods$$v2020$$x1548-7091$$y2019
000427972 7870_ $$0PUBDB-2021-03253$$aPandey, Suraj et.al.$$dLondon [u.a.] : Nature Publishing Group, 2020$$iIsParent$$r$$tDirect Observation of the Mechanism of Antibiotic Resistance by Mix-and-Inject at the European XFEL
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