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@ARTICLE{Pandey:462078,
      author       = {Pandey, Suraj and Bean, Richard and Sato, Tokushi and
                      Poudyal, Ishwor and Bielecki, Johan and Cruz Villarreal,
                      Jorvani and Yefanov, Oleksandr and Mariani, Valerio and
                      White, Thomas A. and Kupitz, Christopher and Hunter, Mark
                      and Abdellatif, Mohamed H. and Bajt, Saša and Bondar,
                      Valerii and Echelmeier, Austin and Doppler, Diandra and
                      Emons, Moritz and Frank, Matthias and Fromme, Raimund and
                      Gevorkov, Yaroslav and Giovanetti, Gabriele and Jiang, Man
                      and Kim, Daihyun and Kim, Yoonhee and Kirkwood, Henry and
                      Klimovskaia, Anna and Knoska, Juraj and Koua, Faisal H. M.
                      and Letrun, Romain and Lisova, Stella and Maia, Luis and
                      Mazalova, Victoria and Meza, Domingo and Michelat, Thomas
                      and Ourmazd, Abbas and Palmer, Guido and Ramilli, Marco and
                      Schubert, Robin and Schwander, Peter and Silenzi, Alessandro
                      and Sztuk-Dambietz, Jolanta and Tolstikova, Alexandra and
                      Chapman, Henry N. and Ros, Alexandra and Barty, Anton and
                      Fromme, Petra and Mancuso, Adrian P. and Schmidt, Marius},
      title        = {{D}irect {O}bservation of the {M}echanism of {A}ntibiotic
                      {R}esistance by {M}ix-and-{I}nject at the {E}uropean {XFEL}},
      publisher    = {biorXiv},
      reportid     = {PUBDB-2021-03253},
      year         = {2020},
      note         = {Published in Nature Methods as: "Pandey, S., Bean, R.,
                      Sato, T. et al. Time-resolved serial femtosecond
                      crystallography at the European XFEL. Nat Methods 17,
                      73–78 (2020). https://doi.org/10.1038/s41592-019-0628-z"},
      abstract     = {In this study, we follow the diffusion and buildup of
                      occupancy of the substrate ceftriaxone in M. tuberculosis
                      β-lactamase BlaC microcrystals by structural analysis of
                      the enzyme substrate complex at single millisecond time
                      resolution. We also show the binding and the reaction of an
                      inhibitor, sulbactam, on a slower millisecond time scale. We
                      use the ‘mix-and-inject’ technique to initiate these
                      reactions by diffusion, and determine the resulting
                      structures by serial crystallography using ultrafast,
                      intense X-ray pulses from the European XFEL (EuXFEL)
                      arriving at MHz repetition rates. Here, we show how to use
                      the EuXFEL pulse structure to dramatically increase the size
                      of the data set and thereby the quality and time resolution
                      of “molecular movies” which unravel ligand binding and
                      enzymatically catalyzed reactions. This shows the great
                      potential for the EuXFEL as a tool for biomedically relevant
                      research, particularly, as shown here, for investigating
                      bacterial antibiotic resistance.},
      cin          = {CFEL-I / FS-CFEL-1 / CFEL-XOM},
      ddc          = {610},
      cid          = {I:(DE-H253)CFEL-I-20161114 / I:(DE-H253)FS-CFEL-1-20120731
                      / I:(DE-H253)CFEL-XOM-20160915},
      pnm          = {6215 - Soft Matter, Health and Life Sciences (POF3-621) /
                      Leibniz Preis - Leibiz Programm 2015: Prof. Dr. Henry N.
                      Chapman (DFG-Leibniz-2015-Chapman) / X-probe - Advanced XFEL
                      and Synchrotron based Probes of Protein Structure and
                      Dynamics (637295) / AXSIS - Frontiers in Attosecond X-ray
                      Science: Imaging and Spectroscopy (609920)},
      pid          = {G:(DE-HGF)POF3-6215 / G:(DE-H253)DFG-Leibniz-2015-Chapman /
                      G:(EU-Grant)637295 / G:(EU-Grant)609920},
      experiment   = {EXP:(DE-H253)XFEL-Exp-20150101},
      typ          = {PUB:(DE-HGF)25},
      doi          = {10.1101/2020.11.24.396689},
      url          = {https://bib-pubdb1.desy.de/record/462078},
}