Journal Article

PUBDB-2019-03696

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Liquid application method for time-resolved analyses by serial synchrotron crystallography

Mehrabi, P. (Corresponding author)CFEL*MPG*EMBL*Extern* ; Schulz, E. C.CFEL*MPG* ; Agthe, M.Extern* ; Horrell, S.Extern* ; Bourenkov, G.EMBL* ; von Stetten, D.EMBL* ; Leimkohl, J.-P.CFEL*EMBL* ; Schikora, H.CFEL*MPG* ; Schneider, T. R.EMBL* ; Pearson, A. R.Extern*EMBL* ; Tellkamp, F.CFEL* ; Miller, R. J. D. (Corresponding author)CFEL*MPG*

2019
Nature Publishing Group London [u.a.]

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Please use a persistent id in citations: doi:10.1038/s41592-019-0553-1

Abstract: We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.

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Contributing Institute(s):

1. Forschungsgruppe für strukturelle Dynamik (MPSD)
2. MPSD (CFEL-ARD)
3. MPSD (CFEL-SSUMP)
4. Arwen Pearson - CUI (CFEL-TRSB)
5. EMBL (EMBL)
6. EMBL-User (EMBL-User)

Research Program(s):

1. 6G3 - PETRA III (POF3-622) (POF3-622)
2. DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)
3. DFG project 194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)

Experiment(s):

1. PETRA Beamline P14 (PETRA III)

Appears in the scientific report 2019

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Database coverage:
; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 25 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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