%0 Journal Article
%A Mehrabi, Pedram
%A Schulz, Eike C.
%A Agthe, Michael
%A Horrell, Sam
%A Bourenkov, Gleb
%A von Stetten, David
%A Leimkohl, Jan-Philipp
%A Schikora, Hendrik
%A Schneider, Thomas R.
%A Pearson, Arwen R.
%A Tellkamp, Friedjof
%A Miller, R. J. Dwayne
%T Liquid application method for time-resolved analyses by serial synchrotron crystallography
%J Nature methods
%V 16
%N 10
%@ 1548-7105
%C London [u.a.]
%I Nature Publishing Group
%M PUBDB-2019-03696
%P 979 - 982
%D 2019
%X We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:31527838
%U <Go to ISI:>//WOS:000488225900025
%R 10.1038/s41592-019-0553-1
%U https://bib-pubdb1.desy.de/record/426351