TY  - JOUR
AU  - Mehrabi, Pedram
AU  - Schulz, Eike C.
AU  - Agthe, Michael
AU  - Horrell, Sam
AU  - Bourenkov, Gleb
AU  - von Stetten, David
AU  - Leimkohl, Jan-Philipp
AU  - Schikora, Hendrik
AU  - Schneider, Thomas R.
AU  - Pearson, Arwen R.
AU  - Tellkamp, Friedjof
AU  - Miller, R. J. Dwayne
TI  - Liquid application method for time-resolved analyses by serial synchrotron crystallography
JO  - Nature methods
VL  - 16
IS  - 10
SN  - 1548-7105
CY  - London [u.a.]
PB  - Nature Publishing Group
M1  - PUBDB-2019-03696
SP  - 979 - 982
PY  - 2019
AB  - We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.
LB  - PUB:(DE-HGF)16
C6  - pmid:31527838
UR  - <Go to ISI:>//WOS:000488225900025
DO  - DOI:10.1038/s41592-019-0553-1
UR  - https://bib-pubdb1.desy.de/record/426351
ER  -