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024 7 _ |a 10.1038/s41592-019-0553-1
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100 1 _ |a Mehrabi, Pedram
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245 _ _ |a Liquid application method for time-resolved analyses by serial synchrotron crystallography
260 _ _ |a London [u.a.]
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520 _ _ |a We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.
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700 1 _ |a Schulz, Eike C.
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700 1 _ |a Agthe, Michael
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700 1 _ |a Horrell, Sam
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700 1 _ |a Bourenkov, Gleb
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700 1 _ |a von Stetten, David
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700 1 _ |a Leimkohl, Jan-Philipp
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700 1 _ |a Schikora, Hendrik
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700 1 _ |a Schneider, Thomas R.
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700 1 _ |a Pearson, Arwen R.
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700 1 _ |a Tellkamp, Friedjof
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700 1 _ |a Miller, R. J. Dwayne
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773 _ _ |a 10.1038/s41592-019-0553-1
|g Vol. 16, no. 10, p. 979 - 982
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