Home > Publications database > Double-flow focused liquid injector for efficient serial femtosecond crystallography
 
Journal Article PUBDB-2017-01394
http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png
Double-flow focused liquid injector for efficient serial femtosecond crystallography

 ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;

2017
Springer Nature London

Scientific reports 7, 44628 () [10.1038/srep44628]
 GO

This record in other databases:        

Please use a persistent id in citations: doi:  doi:

Abstract: Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.

Classification:
Contributing Institute(s):
  1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
  2. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
  3. FS-ML (CFEL-XOM)
  4. MPSD (CFEL-QCM)
Research Program(s):
  1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
  2. AXSIS - Frontiers in Attosecond X-ray Science: Imaging and Spectroscopy (609920) (609920)
  3. NANOMEM - Membrane Protein Nanocrystallography (317079) (317079)
  4. 05K13GU7 - Test eines "Serial Femtosecond Crystallography (SFX)"-Messtandes am Europäischen Elektronenlaser XFEL (BMBF-05K13GU7) (BMBF-05K13GU7)
  5. 05E13GU1 - Entwicklung eines "Serial Femtosecond Crystallography (SFX)"-Messtandes am Europäischen Elektronenlaser XFEL (BMBF-05E13GU1) (BMBF-05E13GU1)
Experiment(s):
  1. PETRA Beamline P05 (PETRA III)

Appears in the scientific report 2017
Database coverage:
Medline ; Creative Commons Attribution CC BY 4.0 ; DOAJ ; OpenAccess ; BIOSIS Previews ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record
Click to display QR Code for this record

The record appears in these collections:
Private Collections > >CFEL > >FS-CFEL > CFEL-XOM
Private Collections > >CFEL > >FS-CFEL > CFEL-I
Private Collections > >CFEL > >MPSD > CFEL-QCM
Private Collections > >DESY > >FS > FS-CFEL-1
Document types > Articles > Journal Article
Public records
Publication Charges
Publications database
OpenAccess
Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;  ;
Corrigendum: Double-flow focused liquid injector for efficient serial femtosecond crystallography
Scientific reports 7, 46846 () [10.1038/srep46846]  GO OpenAccess  Download fulltext Files BibTeX | EndNote: XML, Text | RIS

 Record created 2017-03-16, last modified 2025-07-17
Similar records


OpenAccess:
Download fulltext PDF
Rate this document:

Rate this document:
1
2
3
4
5
 
(Not yet reviewed)
PUBDB :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.7 | join2_v2508a
Maintained by l.pubdb@desy.de

Impressum | Data Privacy Policy