Home > Publications database > Double-flow focused liquid injector for efficient serial femtosecond crystallography > print

001     318779
005     20250717103355.0
024 7 _ |a 10.1038/srep44628
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100 1 _ |a Oberthuer, Dominik
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245 _ _ |a Double-flow focused liquid injector for efficient serial femtosecond crystallography
260 _ _ |a London
|c 2017
|b Springer Nature
336 7 _ |a article
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520 _ _ |a Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.
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536 _ _ |a AXSIS - Frontiers in Attosecond X-ray Science: Imaging and Spectroscopy (609920)
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536 _ _ |a NANOMEM - Membrane Protein Nanocrystallography (317079)
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536 _ _ |a 05K13GU7 - Test eines "Serial Femtosecond Crystallography (SFX)"-Messtandes am Europäischen Elektronenlaser XFEL (BMBF-05K13GU7)
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536 _ _ |a 05E13GU1 - Entwicklung eines "Serial Femtosecond Crystallography (SFX)"-Messtandes am Europäischen Elektronenlaser XFEL (BMBF-05E13GU1)
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700 1 _ |a Knoška, Juraj
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700 1 _ |a Wiedorn, Max O.
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700 1 _ |a Beyerlein, Kenneth
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700 1 _ |a Bushnell, David A.
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700 1 _ |a Kovaleva, Elena G.
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700 1 _ |a Heymann, Michael
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700 1 _ |a Gumprecht, Lars
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700 1 _ |a Kirian, Richard A.
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700 1 _ |a Barty, Anton
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700 1 _ |a Mariani, Valerio
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700 1 _ |a Tolstikova, Aleksandra
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700 1 _ |a Adriano, Luigi
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700 1 _ |a Awel, Salah
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700 1 _ |a Barthelmess, Miriam
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700 1 _ |a Doerner, Katerina
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700 1 _ |a Xavier, P. Lourdu
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700 1 _ |a Yefanov, Oleksandr
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700 1 _ |a James, Daniel R.
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700 1 _ |a Nelson, Garrett
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700 1 _ |a Wang, Dingjie
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700 1 _ |a Calvey, George
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700 1 _ |a Chen, Yujie
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700 1 _ |a Schmidt, Andrea
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700 1 _ |a Szczepek, Michael
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700 1 _ |a Frielingsdorf, Stefan
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700 1 _ |a Lenz, Oliver
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700 1 _ |a Snell, Edward
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700 1 _ |a Robinson, Philip J.
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700 1 _ |a Šarler, Božidar
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700 1 _ |a Belšak, Grega
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700 1 _ |a Maček, Marjan
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700 1 _ |a Wilde, Fabian
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700 1 _ |a Aquila, Andrew
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700 1 _ |a Boutet, Sébastien
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700 1 _ |a Liang, Mengning
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700 1 _ |a Hunter, Mark S.
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700 1 _ |a Scheerer, Patrick
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700 1 _ |a Lipscomb, John D.
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700 1 _ |a Weierstall, Uwe
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700 1 _ |a Kornberg, Roger D.
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700 1 _ |a Spence, John C. H.
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700 1 _ |a Pollack, Lois
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700 1 _ |a Chapman, Henry N.
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700 1 _ |a Bajt, Saša
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773 _ _ |a 10.1038/srep44628
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