%0 Journal Article
%A Oberthuer, Dominik
%A Knoška, Juraj
%A Wiedorn, Max O.
%A Beyerlein, Kenneth
%A Bushnell, David A.
%A Kovaleva, Elena G.
%A Heymann, Michael
%A Gumprecht, Lars
%A Kirian, Richard A.
%A Barty, Anton
%A Mariani, Valerio
%A Tolstikova, Aleksandra
%A Adriano, Luigi
%A Awel, Salah
%A Barthelmess, Miriam
%A Doerner, Katerina
%A Xavier, P. Lourdu
%A Yefanov, Oleksandr
%A James, Daniel R.
%A Nelson, Garrett
%A Wang, Dingjie
%A Calvey, George
%A Chen, Yujie
%A Schmidt, Andrea
%A Szczepek, Michael
%A Frielingsdorf, Stefan
%A Lenz, Oliver
%A Snell, Edward
%A Robinson, Philip J.
%A Šarler, Božidar
%A Belšak, Grega
%A Maček, Marjan
%A Wilde, Fabian
%A Aquila, Andrew
%A Boutet, Sébastien
%A Liang, Mengning
%A Hunter, Mark S.
%A Scheerer, Patrick
%A Lipscomb, John D.
%A Weierstall, Uwe
%A Kornberg, Roger D.
%A Spence, John C. H.
%A Pollack, Lois
%A Chapman, Henry N.
%A Bajt, Saša
%T Double-flow focused liquid injector for efficient serial femtosecond crystallography
%J Scientific reports
%V 7
%@ 2045-2322
%C London
%I Springer Nature
%M PUBDB-2017-01394
%P 44628
%D 2017
%X Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000396544500001
%$ pmid:28300169
%R 10.1038/srep44628
%U https://bib-pubdb1.desy.de/record/318779