TY  - JOUR
AU  - Oberthuer, Dominik
AU  - Knoška, Juraj
AU  - Wiedorn, Max O.
AU  - Beyerlein, Kenneth
AU  - Bushnell, David A.
AU  - Kovaleva, Elena G.
AU  - Heymann, Michael
AU  - Gumprecht, Lars
AU  - Kirian, Richard A.
AU  - Barty, Anton
AU  - Mariani, Valerio
AU  - Tolstikova, Aleksandra
AU  - Adriano, Luigi
AU  - Awel, Salah
AU  - Barthelmess, Miriam
AU  - Doerner, Katerina
AU  - Xavier, P. Lourdu
AU  - Yefanov, Oleksandr
AU  - James, Daniel R.
AU  - Nelson, Garrett
AU  - Wang, Dingjie
AU  - Calvey, George
AU  - Chen, Yujie
AU  - Schmidt, Andrea
AU  - Szczepek, Michael
AU  - Frielingsdorf, Stefan
AU  - Lenz, Oliver
AU  - Snell, Edward
AU  - Robinson, Philip J.
AU  - Šarler, Božidar
AU  - Belšak, Grega
AU  - Maček, Marjan
AU  - Wilde, Fabian
AU  - Aquila, Andrew
AU  - Boutet, Sébastien
AU  - Liang, Mengning
AU  - Hunter, Mark S.
AU  - Scheerer, Patrick
AU  - Lipscomb, John D.
AU  - Weierstall, Uwe
AU  - Kornberg, Roger D.
AU  - Spence, John C. H.
AU  - Pollack, Lois
AU  - Chapman, Henry N.
AU  - Bajt, Saša
TI  - Double-flow focused liquid injector for efficient serial femtosecond crystallography
JO  - Scientific reports
VL  - 7
SN  - 2045-2322
CY  - London
PB  - Springer Nature
M1  - PUBDB-2017-01394
SP  - 44628
PY  - 2017
AB  - Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000396544500001
C6  - pmid:28300169
DO  - DOI:10.1038/srep44628
UR  - https://bib-pubdb1.desy.de/record/318779
ER  -