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@ARTICLE{Oberthuer:318779,
      author       = {Oberthuer, Dominik and Knoška, Juraj and Wiedorn, Max O.
                      and Beyerlein, Kenneth and Bushnell, David A. and Kovaleva,
                      Elena G. and Heymann, Michael and Gumprecht, Lars and
                      Kirian, Richard A. and Barty, Anton and Mariani, Valerio and
                      Tolstikova, Aleksandra and Adriano, Luigi and Awel, Salah
                      and Barthelmess, Miriam and Doerner, Katerina and Xavier, P.
                      Lourdu and Yefanov, Oleksandr and James, Daniel R. and
                      Nelson, Garrett and Wang, Dingjie and Calvey, George and
                      Chen, Yujie and Schmidt, Andrea and Szczepek, Michael and
                      Frielingsdorf, Stefan and Lenz, Oliver and Snell, Edward and
                      Robinson, Philip J. and Šarler, Božidar and Belšak, Grega
                      and Maček, Marjan and Wilde, Fabian and Aquila, Andrew and
                      Boutet, Sébastien and Liang, Mengning and Hunter, Mark S.
                      and Scheerer, Patrick and Lipscomb, John D. and Weierstall,
                      Uwe and Kornberg, Roger D. and Spence, John C. H. and
                      Pollack, Lois and Chapman, Henry N. and Bajt, Saša},
      title        = {{D}ouble-flow focused liquid injector for efficient serial
                      femtosecond crystallography},
      journal      = {Scientific reports},
      volume       = {7},
      issn         = {2045-2322},
      address      = {London},
      publisher    = {Springer Nature},
      reportid     = {PUBDB-2017-01394},
      pages        = {44628},
      year         = {2017},
      abstract     = {Serial femtosecond crystallography requires reliable and
                      efficient delivery of fresh crystals across the beam of an
                      X-ray free-electron laser over the course of an experiment.
                      We introduce a double-flow focusing nozzle to meet this
                      challenge, with significantly reduced sample consumption,
                      while improving jet stability over previous generations of
                      nozzles. We demonstrate its use to determine the first
                      room-temperature structure of RNA polymerase II at high
                      resolution, revealing new structural details. Moreover, the
                      double flow-focusing nozzles were successfully tested with
                      three other protein samples and the first room temperature
                      structure of an extradiol ring-cleaving dioxygenase was
                      solved by utilizing the improved operation and
                      characteristics of these devices.},
      cin          = {FS-CFEL-1 / CFEL-I / CFEL-XOM / CFEL-QCM},
      ddc          = {000},
      cid          = {I:(DE-H253)FS-CFEL-1-20120731 / I:(DE-H253)CFEL-I-20161114
                      / I:(DE-H253)CFEL-XOM-20160915 /
                      I:(DE-H253)CFEL-QCM-20160914},
      pnm          = {6215 - Soft Matter, Health and Life Sciences (POF3-621) /
                      AXSIS - Frontiers in Attosecond X-ray Science: Imaging and
                      Spectroscopy (609920) / NANOMEM - Membrane Protein
                      Nanocrystallography (317079) / 05K13GU7 - Test eines "Serial
                      Femtosecond Crystallography (SFX)"-Messtandes am
                      Europäischen Elektronenlaser XFEL (BMBF-05K13GU7) /
                      05E13GU1 - Entwicklung eines "Serial Femtosecond
                      Crystallography (SFX)"-Messtandes am Europäischen
                      Elektronenlaser XFEL (BMBF-05E13GU1)},
      pid          = {G:(DE-HGF)POF3-6215 / G:(EU-Grant)609920 /
                      G:(EU-Grant)317079 / G:(DE-H253)BMBF-05K13GU7 /
                      G:(DE-H253)BMBF-05E13GU1},
      experiment   = {EXP:(DE-H253)P-P05-20150101},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000396544500001},
      pubmed       = {pmid:28300169},
      doi          = {10.1038/srep44628},
      url          = {https://bib-pubdb1.desy.de/record/318779},
}