Journal Article (Erratum/Correction)

PUBDB-2018-00518

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Corrigendum: Double-flow focused liquid injector for efficient serial femtosecond crystallography

Oberthuer, D. (Corresponding author)CFEL*DESY* ; Knoška, J.CFEL*XFEL.EU* ; Wiedorn, M. O.CFEL* ; Beyerlein, K. R. ; Bushnell, D. A. ; Kovaleva, E. G. ; Heymann, M.Extern* ; Gumprecht, L.CFEL*DESY* ; Kirian, R. A. ; Barty, A.CFEL*DESY* ; Mariani, V.CFEL*DESY* ; Tolstikova, A.CFEL*DESY* ; Adriano, L.CFEL*DESY* ; Awel, S.CFEL* ; Barthelmess, M.CFEL*DESY* ; Dörner, K.XFEL.EU* ; Xavier, P. L.CFEL* ; Yefanov, O.CFEL*EMBL*DESY* ; James, D. R. ; Nelson, G.Extern* ; Wang, D. ; Calvey, G.Extern* ; Chen, Y. ; Schmidt, A. ; Szczepek, M. ; Frielingsdorf, S. ; Lenz, O. ; Snell, E. ; Robinson, P. J. ; Šarler, B. ; Belšak, G. ; Maček, M. ; Wilde, F.HZG* ; Aquila, A.Extern* ; Boutet, S.Extern* ; Liang, M.CFEL*DESY* ; Hunter, M. S. ; Scheerer, P.Extern* ; Lipscomb, J. D. ; Weierstall, U.Extern* ; Kornberg, R. D. ; Spence, J. C. H. ; Pollack, L.Extern* ; Chapman, H. N.CFEL*DESY* ; Bajt, S.CFEL*DESY*

2017
Nature Publishing Group London

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Please use a persistent id in citations: doi:10.1038/srep46846  doi:10.3204/PUBDB-2018-00518

Abstract: Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
2. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)

Experiment(s):

1. Measurement at external facility

Appears in the scientific report 2017

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Database coverage:
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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Oberthuer, D.CFEL*DESY* ; Knoška, J.CFEL*DESY* ; Wiedorn, M. O.CFEL*DESY* ; Beyerlein, K.CFEL*MPG* ; Bushnell, D. A. ; Kovaleva, E. G. ; Heymann, M. ; Gumprecht, L.CFEL*DESY* ; Kirian, R. A. ; Barty, A.CFEL*DESY* ; Mariani, V.CFEL*DESY* ; Tolstikova, A.CFEL*DESY* ; Adriano, L.CFEL*DESY* ; Awel, S.CFEL*DESY* ; Barthelmess, M.CFEL*DESY* ; Doerner, K.CFEL*XFEL.EU* ; Xavier, P. L. ; Yefanov, O. ; James, D. R. ; Nelson, G. ; Wang, D. ; Calvey, G. ; Chen, Y. ; Schmidt, A. ; Szczepek, M. ; Frielingsdorf, S. ; Lenz, O. ; Snell, E. ; Robinson, P. J. ; Šarler, B. ; Belšak, G. ; Maček, M. ; Wilde, F.HZG* ; Aquila, A. ; Boutet, S. ; Liang, M.CFEL*DESY* ; Hunter, M. S. ; Scheerer, P. ; Lipscomb, J. D. ; Weierstall, U. ; Kornberg, R. D. ; Spence, J. C. H. ; Pollack, L. ; Chapman, H. N.CFEL*DESY* ; Bajt, S. (Corresponding author)CFEL*DESY*
Double-flow focused liquid injector for efficient serial femtosecond crystallography
Scientific reports 7, 44628 (2017) [10.1038/srep44628]2017     Files BibTeX | EndNote: XML, Text | RIS

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