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| 001 | 399213 | ||
| 005 | 20260204122250.0 | ||
| 024 | 7 | _ | |a 10.1038/srep46846 |2 doi |
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| 082 | _ | _ | |a 000 |
| 100 | 1 | _ | |a Oberthuer, Dominik |0 P:(DE-H253)PIP1012087 |b 0 |e Corresponding author |
| 245 | _ | _ | |a Corrigendum: Double-flow focused liquid injector for efficient serial femtosecond crystallography |
| 260 | _ | _ | |a London |c 2017 |b Nature Publishing Group |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices. |
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| 773 | _ | _ | |a 10.1038/srep46846 |g Vol. 7, p. 46846 - |0 PERI:(DE-600)2615211-3 |p 46846 |t Scientific reports |v 7 |y 2017 |x 2045-2322 |
| 787 | 0 | _ | |a Oberthuer, Dominik et.al. |d London : Springer Nature, 2017 |i IsPartOf |0 PUBDB-2017-01394 |r |t Double-flow focused liquid injector for efficient serial femtosecond crystallography |
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