Journal Article

PUBDB-2016-03927

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Nogly, P. ; Panneels, V. ; Nelson, G.Extern* ; Gati, C.CFEL*EMBL* ; Kimura, T. ; Milne, C. ; Milathianaki, D. ; Kubo, M. ; Wu, W. ; Conrad, C.Extern* ; Coe, J.Extern* ; Bean, R.XFEL.EU* ; Zhao, Y. ; Båth, P. ; Dods, R. ; Harimoorthy, R.Extern* ; Beyerlein, K.CFEL*DESY* ; Rheinberger, J. ; James, D. ; DePonte, D. ; Li, C. ; Sala, L. ; Williams, G. J. ; Hunter, M. S. ; Koglin, J. E. ; Berntsen, P. ; Nango, E. ; Iwata, S. ; Chapman, H. N.CFEL*DESY* ; Fromme, P.Extern* ; Frank, M. ; Abela, R. ; Boutet, S.Extern* ; Barty, A.CFEL*DESY* ; White, T.CFEL*DESY* ; Weierstall, U.Extern* ; Spence, J.Extern* ; Neutze, R. ; Schertler, G. ; Standfuss, J. (Corresponding author)

2016
Nature Publishing Group London

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Please use a persistent id in citations: doi:10.1038/ncomms12314  doi:10.3204/PUBDB-2016-03927

Abstract: Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. NANOMEM - Membrane Protein Nanocrystallography (317079) (317079)
3. PSI-FELLOW - International Fellowship Program on Materials & Matter, Energy & Environment, Human Health & Life-Sciences, and Accelerator Technology (290605) (290605)
4. VH-GS-500 - PIER Helmholtz Graduate School (2015_IFV-VH-GS-500) (2015_IFV-VH-GS-500)

Experiment(s):

1. Experiments at CFEL
2. Measurement at external facility

Appears in the scientific report 2016

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Database coverage:
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