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| 001 | 309582 | ||
| 005 | 20250717104507.0 | ||
| 024 | 7 | _ | |a 10.1038/ncomms12314 |2 doi |
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| 100 | 1 | _ | |a Nogly, Przemyslaw |0 0000-0002-8040-7753 |b 0 |
| 245 | _ | _ | |a Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography |
| 260 | _ | _ | |a London |c 2016 |b Nature Publishing Group |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 520 | _ | _ | |a Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX. |
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