TY  - JOUR
AU  - Nogly, Przemyslaw
AU  - Panneels, Valerie
AU  - Nelson, Garrett
AU  - Gati, Cornelius
AU  - Kimura, Tetsunari
AU  - Milne, Christopher
AU  - Milathianaki, Despina
AU  - Kubo, Minoru
AU  - Wu, Wenting
AU  - Conrad, Chelsie
AU  - Coe, Jesse
AU  - Bean, Richard
AU  - Zhao, Yun
AU  - Båth, Petra
AU  - Dods, Robert
AU  - Harimoorthy, Rajiv
AU  - Beyerlein, Kenneth
AU  - Rheinberger, Jan
AU  - James, Daniel
AU  - DePonte, Daniel
AU  - Li, Chufeng
AU  - Sala, Leonardo
AU  - Williams, Garth J.
AU  - Hunter, Mark S.
AU  - Koglin, Jason E.
AU  - Berntsen, Peter
AU  - Nango, Eriko
AU  - Iwata, So
AU  - Chapman, Henry N.
AU  - Fromme, Petra
AU  - Frank, Matthias
AU  - Abela, Rafael
AU  - Boutet, Sébastien
AU  - Barty, Anton
AU  - White, Thomas
AU  - Weierstall, Uwe
AU  - Spence, John
AU  - Neutze, Richard
AU  - Schertler, Gebhard
AU  - Standfuss, Jörg
TI  - Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
JO  - Nature Communications
VL  - 7
SN  - 2041-1723
CY  - London
PB  - Nature Publishing Group
M1  - PUBDB-2016-03927
SP  - 12314
PY  - 2016
AB  - Serial  femtosecond  crystallography  (SFX)  using  X-ray  free-electron  laser  sources  is  an emerging method with considerable potential for time-resolved pump-probe experiments. Here  we  present  a  lipidic  cubic  phase  SFX  structure  of  the  light-driven  proton  pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest  sample  requirement.  Time-resolved  SFX  (TR-SFX)  with  a  pump-probe  delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step  towards  characterizing  the  complete  photocycle  dynamics  of  retinal  proteins  and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000381904200001
C6  - pmid:27545823
DO  - DOI:10.1038/ncomms12314
UR  - https://bib-pubdb1.desy.de/record/309582
ER  -