%0 Journal Article
%A Nogly, Przemyslaw
%A Panneels, Valerie
%A Nelson, Garrett
%A Gati, Cornelius
%A Kimura, Tetsunari
%A Milne, Christopher
%A Milathianaki, Despina
%A Kubo, Minoru
%A Wu, Wenting
%A Conrad, Chelsie
%A Coe, Jesse
%A Bean, Richard
%A Zhao, Yun
%A Båth, Petra
%A Dods, Robert
%A Harimoorthy, Rajiv
%A Beyerlein, Kenneth
%A Rheinberger, Jan
%A James, Daniel
%A DePonte, Daniel
%A Li, Chufeng
%A Sala, Leonardo
%A Williams, Garth J.
%A Hunter, Mark S.
%A Koglin, Jason E.
%A Berntsen, Peter
%A Nango, Eriko
%A Iwata, So
%A Chapman, Henry N.
%A Fromme, Petra
%A Frank, Matthias
%A Abela, Rafael
%A Boutet, Sébastien
%A Barty, Anton
%A White, Thomas
%A Weierstall, Uwe
%A Spence, John
%A Neutze, Richard
%A Schertler, Gebhard
%A Standfuss, Jörg
%T Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
%J Nature Communications
%V 7
%@ 2041-1723
%C London
%I Nature Publishing Group
%M PUBDB-2016-03927
%P 12314
%D 2016
%X Serial  femtosecond  crystallography  (SFX)  using  X-ray  free-electron  laser  sources  is  an emerging method with considerable potential for time-resolved pump-probe experiments. Here  we  present  a  lipidic  cubic  phase  SFX  structure  of  the  light-driven  proton  pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest  sample  requirement.  Time-resolved  SFX  (TR-SFX)  with  a  pump-probe  delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step  towards  characterizing  the  complete  photocycle  dynamics  of  retinal  proteins  and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000381904200001
%$ pmid:27545823
%R 10.1038/ncomms12314
%U https://bib-pubdb1.desy.de/record/309582