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000309582 1001_ $$00000-0002-8040-7753$$aNogly, Przemyslaw$$b0
000309582 245__ $$aLipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
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000309582 520__ $$aSerial  femtosecond  crystallography  (SFX)  using  X-ray  free-electron  laser  sources  is  an emerging method with considerable potential for time-resolved pump-probe experiments. Here  we  present  a  lipidic  cubic  phase  SFX  structure  of  the  light-driven  proton  pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest  sample  requirement.  Time-resolved  SFX  (TR-SFX)  with  a  pump-probe  delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step  towards  characterizing  the  complete  photocycle  dynamics  of  retinal  proteins  and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
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000309582 536__ $$0G:(DE-HGF)2015_IFV-VH-GS-500$$aVH-GS-500 - PIER Helmholtz Graduate School (2015_IFV-VH-GS-500)$$c2015_IFV-VH-GS-500$$x3
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000309582 7001_ $$0P:(DE-HGF)0$$aPanneels, Valerie$$b1
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000309582 7001_ $$00000-0003-4714-9139$$aMilne, Christopher$$b5
000309582 7001_ $$0P:(DE-HGF)0$$aMilathianaki, Despina$$b6
000309582 7001_ $$0P:(DE-HGF)0$$aKubo, Minoru$$b7
000309582 7001_ $$0P:(DE-HGF)0$$aWu, Wenting$$b8
000309582 7001_ $$0P:(DE-H253)PIP1027131$$aConrad, Chelsie$$b9
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000309582 7001_ $$0P:(DE-H253)PIP1016993$$aBean, Richard$$b11
000309582 7001_ $$0P:(DE-HGF)0$$aZhao, Yun$$b12
000309582 7001_ $$0P:(DE-HGF)0$$aBåth, Petra$$b13
000309582 7001_ $$0P:(DE-HGF)0$$aDods, Robert$$b14
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000309582 7001_ $$0P:(DE-H253)PIP1016189$$aBeyerlein, Kenneth$$b16$$udesy
000309582 7001_ $$0P:(DE-HGF)0$$aRheinberger, Jan$$b17
000309582 7001_ $$00000-0002-8348-6661$$aJames, Daniel$$b18
000309582 7001_ $$0P:(DE-HGF)0$$aDePonte, Daniel$$b19
000309582 7001_ $$0P:(DE-HGF)0$$aLi, Chufeng$$b20
000309582 7001_ $$0P:(DE-HGF)0$$aSala, Leonardo$$b21
000309582 7001_ $$0P:(DE-HGF)0$$aWilliams, Garth J.$$b22
000309582 7001_ $$0P:(DE-HGF)0$$aHunter, Mark S.$$b23
000309582 7001_ $$00000-0001-6811-6083$$aKoglin, Jason E.$$b24
000309582 7001_ $$0P:(DE-HGF)0$$aBerntsen, Peter$$b25
000309582 7001_ $$0P:(DE-HGF)0$$aNango, Eriko$$b26
000309582 7001_ $$0P:(DE-HGF)0$$aIwata, So$$b27
000309582 7001_ $$0P:(DE-H253)PIP1006324$$aChapman, Henry N.$$b28
000309582 7001_ $$0P:(DE-H253)PIP1023170$$aFromme, Petra$$b29
000309582 7001_ $$00000-0002-5244-0791$$aFrank, Matthias$$b30
000309582 7001_ $$0P:(DE-HGF)0$$aAbela, Rafael$$b31
000309582 7001_ $$0P:(DE-H253)PIP1009086$$aBoutet, Sébastien$$b32
000309582 7001_ $$0P:(DE-H253)PIP1008245$$aBarty, Anton$$b33$$udesy
000309582 7001_ $$0P:(DE-H253)PIP1010779$$aWhite, Thomas$$b34$$udesy
000309582 7001_ $$0P:(DE-H253)PIP1008265$$aWeierstall, Uwe$$b35
000309582 7001_ $$0P:(DE-H253)PIP1008263$$aSpence, John$$b36
000309582 7001_ $$0P:(DE-HGF)0$$aNeutze, Richard$$b37
000309582 7001_ $$0P:(DE-HGF)0$$aSchertler, Gebhard$$b38
000309582 7001_ $$0P:(DE-HGF)0$$aStandfuss, Jörg$$b39$$eCorresponding author
000309582 773__ $$0PERI:(DE-600)2553671-0$$a10.1038/ncomms12314$$gVol. 7, p. 12314 -$$p12314$$tNature Communications$$v7$$x2041-1723$$y2016
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