Journal Article

PUBDB-2025-02617

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png X-ray phase-contrast tomography of cells manipulated with an optical stretcher

Burchert, J.-P.Extern* ; Frohn, J.Extern* ; Rölleke, U. ; Bruns, H.Extern* ; Yu, B.Extern* ; Gleber, S.-C. ; Stange, R. ; Busse, M.Extern* ; Osterhoff, M.Extern*XFEL.EU* ; Salditt, T.Extern* ; Koester, S. (Corresponding author)Extern*

2024
IUCr Chester

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Please use a persistent id in citations: doi:10.1107/S1600577524003618  doi:10.3204/PUBDB-2025-02617

Abstract: X-rays can penetrate deeply into biological cells and thus allow for examination of their internal structures with high spatial resolution. In this study, X-ray phase-contrast imaging and tomography is combined with an X-ray-compatible optical stretcher and microfluidic sample delivery. Using this setup, individual cells can be kept in suspension while they are examined with the X-ray beam at a synchrotron. From the recorded holograms, 2D phase shift images that are proportional to the projected local electron density of the investigated cell can be calculated. From the tomographic reconstruction of multiple such projections the 3D electron density can be obtained. The cells can thus be studied in a hydrated or even living state, thus avoiding artifacts from freezing, drying or embedding, and can in principle also be subjected to different sample environments or mechanical strains. This combination of techniques is applied to living as well as fixed and stained NIH3T3 mouse fibroblasts and the effect of the beam energy on the phase shifts is investigated. Furthermore, a 3D algebraic reconstruction scheme and a dedicated mathematical description is used to follow the motion of the trapped cells in the optical stretcher for multiple rotations.

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Contributing Institute(s):

1. DOOR-User (DOOR ; HAS-User)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
2. DFG project G:(GEPRIS)390729940 - EXC 2067: Multiscale Bioimaging: Von molekularen Maschinen zu Netzwerken erregbarer Zellen (390729940) (390729940)
3. DFG project G:(GEPRIS)429958739 - Alternative Assemblierungs-Mechanismen von pathologischen Desmin-Mutanten: Filamentbildung im Wettbewerb mit Aggregation (429958739) (429958739)
4. DFG project G:(GEPRIS)460248799 - DAPHNE4NFDI - DAten aus PHoton- und Neutronen Experimenten für NFDI (460248799) (460248799)
5. 05K22MG3 - FastScan: schnelle, korrelative Abbildung biologischer Materie mit scanning SAXS und optischer Fluoreszenzmikroskopie. (BMBF-05K22MG3) (BMBF-05K22MG3)

Experiment(s):

1. PETRA Beamline P10 (PETRA III)

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