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@ARTICLE{Burchert:634793,
      author       = {Burchert, Jan-Philipp and Frohn, Jasper and Rölleke,
                      Ulrike and Bruns, Hendrik and Yu, Boram and Gleber,
                      Sophie-Charlotte and Stange, Roland and Busse, Madleen and
                      Osterhoff, Markus and Salditt, Tim and Koester, Sarah},
      title        = {{X}-ray phase-contrast tomography of cells manipulated with
                      an optical stretcher},
      journal      = {Journal of synchrotron radiation},
      volume       = {31},
      number       = {4},
      issn         = {0909-0495},
      address      = {Chester},
      publisher    = {IUCr},
      reportid     = {PUBDB-2025-02617},
      pages        = {923 - 935},
      year         = {2024},
      abstract     = {X-rays can penetrate deeply into biological cells and thus
                      allow for examination of their internal structures with high
                      spatial resolution. In this study, X-ray phase-contrast
                      imaging and tomography is combined with an X-ray-compatible
                      optical stretcher and microfluidic sample delivery. Using
                      this setup, individual cells can be kept in suspension while
                      they are examined with the X-ray beam at a synchrotron. From
                      the recorded holograms, 2D phase shift images that are
                      proportional to the projected local electron density of the
                      investigated cell can be calculated. From the tomographic
                      reconstruction of multiple such projections the 3D electron
                      density can be obtained. The cells can thus be studied in a
                      hydrated or even living state, thus avoiding artifacts from
                      freezing, drying or embedding, and can in principle also be
                      subjected to different sample environments or mechanical
                      strains. This combination of techniques is applied to living
                      as well as fixed and stained NIH3T3 mouse fibroblasts and
                      the effect of the beam energy on the phase shifts is
                      investigated. Furthermore, a 3D algebraic reconstruction
                      scheme and a dedicated mathematical description is used to
                      follow the motion of the trapped cells in the optical
                      stretcher for multiple rotations.},
      cin          = {DOOR ; HAS-User},
      ddc          = {550},
      cid          = {I:(DE-H253)HAS-User-20120731},
      pnm          = {6G3 - PETRA III (DESY) (POF4-6G3) / DFG project
                      G:(GEPRIS)390729940 - EXC 2067: Multiscale Bioimaging: Von
                      molekularen Maschinen zu Netzwerken erregbarer Zellen
                      (390729940) / DFG project G:(GEPRIS)429958739 - Alternative
                      Assemblierungs-Mechanismen von pathologischen
                      Desmin-Mutanten: Filamentbildung im Wettbewerb mit
                      Aggregation (429958739) / DFG project G:(GEPRIS)460248799 -
                      DAPHNE4NFDI - DAten aus PHoton- und Neutronen Experimenten
                      für NFDI (460248799) / 05K22MG3 - FastScan: schnelle,
                      korrelative Abbildung biologischer Materie mit scanning SAXS
                      und optischer Fluoreszenzmikroskopie. (BMBF-05K22MG3)},
      pid          = {G:(DE-HGF)POF4-6G3 / G:(GEPRIS)390729940 /
                      G:(GEPRIS)429958739 / G:(GEPRIS)460248799 /
                      G:(DE-Ds200)BMBF-05K22MG3},
      experiment   = {EXP:(DE-H253)P-P10-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38861370},
      doi          = {10.1107/S1600577524003618},
      url          = {https://bib-pubdb1.desy.de/record/634793},
}