TY  - JOUR
AU  - Burchert, Jan-Philipp
AU  - Frohn, Jasper
AU  - Rölleke, Ulrike
AU  - Bruns, Hendrik
AU  - Yu, Boram
AU  - Gleber, Sophie-Charlotte
AU  - Stange, Roland
AU  - Busse, Madleen
AU  - Osterhoff, Markus
AU  - Salditt, Tim
AU  - Koester, Sarah
TI  - X-ray phase-contrast tomography of cells manipulated with an optical stretcher
JO  - Journal of synchrotron radiation
VL  - 31
IS  - 4
SN  - 0909-0495
CY  - Chester
PB  - IUCr
M1  - PUBDB-2025-02617
SP  - 923 - 935
PY  - 2024
AB  - X-rays can penetrate deeply into biological cells and thus allow for examination of their internal structures with high spatial resolution. In this study, X-ray phase-contrast imaging and tomography is combined with an X-ray-compatible optical stretcher and microfluidic sample delivery. Using this setup, individual cells can be kept in suspension while they are examined with the X-ray beam at a synchrotron. From the recorded holograms, 2D phase shift images that are proportional to the projected local electron density of the investigated cell can be calculated. From the tomographic reconstruction of multiple such projections the 3D electron density can be obtained. The cells can thus be studied in a hydrated or even living state, thus avoiding artifacts from freezing, drying or embedding, and can in principle also be subjected to different sample environments or mechanical strains. This combination of techniques is applied to living as well as fixed and stained NIH3T3 mouse fibroblasts and the effect of the beam energy on the phase shifts is investigated. Furthermore, a 3D algebraic reconstruction scheme and a dedicated mathematical description is used to follow the motion of the trapped cells in the optical stretcher for multiple rotations. 
LB  - PUB:(DE-HGF)16
C6  - pmid:38861370
DO  - DOI:10.1107/S1600577524003618
UR  - https://bib-pubdb1.desy.de/record/634793
ER  -