Journal Article

PUBDB-2024-05767

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The cell cycle controls spindle architecture in Arabidopsis by activating the augmin pathway

Romeiro Motta, M. ; Nédélec, F. ; Saville, H. ; Woelken, E. ; Jacquerie, C. ; Pastuglia, M. ; Stolze, S. C. ; Van De Slijke, E. ; Böttger, L. ; Belcram, K. ; Nakagami, H. ; De Jaeger, G. ; Bouchez, D. ; Schnittger, A. (Corresponding author)

2024
Elsevier New York, NY

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Please use a persistent id in citations: doi:10.1016/j.devcel.2024.08.001  doi:10.3204/PUBDB-2024-05767

Abstract: To ensure an even segregation of chromosomes during somatic cell division, eukaryotes rely on mitotic spin-dles. Here, we measured prime characteristics of the Arabidopsis mitotic spindle and built a three-dimen-sional dynamic model using Cytosim. We identified the cell-cycle regulator CYCLIN-DEPENDENT KINASEB1 (CDKB1) together with its cyclin partner CYCB3;1 as key regulators of spindle morphology in Arabidopsis.We found that the augmin component ENDOSPERM DEFECTIVE1 (EDE1) is a substrate of the CDKB1;1-CYCB3;1 complex. A non-phosphorylatable mutant rescue of ede1 resembled the spindle phenotypesof cycb3;1 and cdkb1 mutants and the protein associated less efficiently with spindle microtubules. Accord-ingly, reducing the level of augmin in simulations recapitulated the phenotypes observed in the mutants. Ourfindings emphasize the importance of cell-cycle-dependent phospho-control of the mitotic spindle in plantcells and support the validity of our model as a framework for the exploration of mechanisms controllingthe organization of the eukaryotic spindle.

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Contributing Institute(s):

1. CSSB-CF-ALFM (CSSB-CF-ALFM)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Experiment(s):

1. (Advanced Light and Fluorescence Microscopy Facility)

Appears in the scientific report 2024

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 10 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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