%0 Journal Article
%A Romeiro Motta, Mariana
%A Nédélec, François
%A Saville, Helen
%A Woelken, Elke
%A Jacquerie, Claire
%A Pastuglia, Martine
%A Stolze, Sara Christina
%A Van De Slijke, Eveline
%A Böttger, Lev
%A Belcram, Katia
%A Nakagami, Hirofumi
%A De Jaeger, Geert
%A Bouchez, David
%A Schnittger, Arp
%T The cell cycle controls spindle architecture in Arabidopsis by activating the augmin pathway
%J Developmental cell
%V 59
%N 22
%@ 1534-5807
%C New York, NY
%I Elsevier
%M PUBDB-2024-05767
%P 2947-2961.e9
%D 2024
%X To ensure an even segregation of chromosomes during somatic cell division, eukaryotes rely on mitotic spin-dles. Here, we measured prime characteristics of the Arabidopsis mitotic spindle and built a three-dimen-sional dynamic model using Cytosim. We identified the cell-cycle regulator CYCLIN-DEPENDENT KINASEB1 (CDKB1) together with its cyclin partner CYCB3;1 as key regulators of spindle morphology in Arabidopsis.We found that the augmin component ENDOSPERM DEFECTIVE1 (EDE1) is a substrate of the CDKB1;1-CYCB3;1 complex. A non-phosphorylatable mutant rescue of ede1 resembled the spindle phenotypesof cycb3;1 and cdkb1 mutants and the protein associated less efficiently with spindle microtubules. Accord-ingly, reducing the level of augmin in simulations recapitulated the phenotypes observed in the mutants. Ourfindings emphasize the importance of cell-cycle-dependent phospho-control of the mitotic spindle in plantcells and support the validity of our model as a framework for the exploration of mechanisms controllingthe organization of the eukaryotic spindle.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:39191252
%U <Go to ISI:>//WOS:001364262100001
%R 10.1016/j.devcel.2024.08.001
%U https://bib-pubdb1.desy.de/record/614227