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@ARTICLE{RomeiroMotta:614227,
author = {Romeiro Motta, Mariana and Nédélec, François and
Saville, Helen and Woelken, Elke and Jacquerie, Claire and
Pastuglia, Martine and Stolze, Sara Christina and Van De
Slijke, Eveline and Böttger, Lev and Belcram, Katia and
Nakagami, Hirofumi and De Jaeger, Geert and Bouchez, David
and Schnittger, Arp},
title = {{T}he cell cycle controls spindle architecture in
{A}rabidopsis by activating the augmin pathway},
journal = {Developmental cell},
volume = {59},
number = {22},
issn = {1534-5807},
address = {New York, NY},
publisher = {Elsevier},
reportid = {PUBDB-2024-05767},
pages = {2947-2961.e9},
year = {2024},
abstract = {To ensure an even segregation of chromosomes during somatic
cell division, eukaryotes rely on mitotic spin-dles. Here,
we measured prime characteristics of the Arabidopsis mitotic
spindle and built a three-dimen-sional dynamic model using
Cytosim. We identified the cell-cycle regulator
CYCLIN-DEPENDENT KINASEB1 (CDKB1) together with its cyclin
partner CYCB3;1 as key regulators of spindle morphology in
Arabidopsis.We found that the augmin component ENDOSPERM
DEFECTIVE1 (EDE1) is a substrate of the CDKB1;1-CYCB3;1
complex. A non-phosphorylatable mutant rescue of ede1
resembled the spindle phenotypesof cycb3;1 and cdkb1 mutants
and the protein associated less efficiently with spindle
microtubules. Accord-ingly, reducing the level of augmin in
simulations recapitulated the phenotypes observed in the
mutants. Ourfindings emphasize the importance of
cell-cycle-dependent phospho-control of the mitotic spindle
in plantcells and support the validity of our model as a
framework for the exploration of mechanisms controllingthe
organization of the eukaryotic spindle.},
cin = {CSSB-CF-ALFM},
ddc = {610},
cid = {I:(DE-H253)CSSB-CF-ALFM-20210629},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
experiment = {EXP:(DE-H253)ALFM-20250101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39191252},
UT = {WOS:001364262100001},
doi = {10.1016/j.devcel.2024.08.001},
url = {https://bib-pubdb1.desy.de/record/614227},
}