TY  - JOUR
AU  - Romeiro Motta, Mariana
AU  - Nédélec, François
AU  - Saville, Helen
AU  - Woelken, Elke
AU  - Jacquerie, Claire
AU  - Pastuglia, Martine
AU  - Stolze, Sara Christina
AU  - Van De Slijke, Eveline
AU  - Böttger, Lev
AU  - Belcram, Katia
AU  - Nakagami, Hirofumi
AU  - De Jaeger, Geert
AU  - Bouchez, David
AU  - Schnittger, Arp
TI  - The cell cycle controls spindle architecture in Arabidopsis by activating the augmin pathway
JO  - Developmental cell
VL  - 59
IS  - 22
SN  - 1534-5807
CY  - New York, NY
PB  - Elsevier
M1  - PUBDB-2024-05767
SP  - 2947-2961.e9
PY  - 2024
AB  - To ensure an even segregation of chromosomes during somatic cell division, eukaryotes rely on mitotic spin-dles. Here, we measured prime characteristics of the Arabidopsis mitotic spindle and built a three-dimen-sional dynamic model using Cytosim. We identified the cell-cycle regulator CYCLIN-DEPENDENT KINASEB1 (CDKB1) together with its cyclin partner CYCB3;1 as key regulators of spindle morphology in Arabidopsis.We found that the augmin component ENDOSPERM DEFECTIVE1 (EDE1) is a substrate of the CDKB1;1-CYCB3;1 complex. A non-phosphorylatable mutant rescue of ede1 resembled the spindle phenotypesof cycb3;1 and cdkb1 mutants and the protein associated less efficiently with spindle microtubules. Accord-ingly, reducing the level of augmin in simulations recapitulated the phenotypes observed in the mutants. Ourfindings emphasize the importance of cell-cycle-dependent phospho-control of the mitotic spindle in plantcells and support the validity of our model as a framework for the exploration of mechanisms controllingthe organization of the eukaryotic spindle.
LB  - PUB:(DE-HGF)16
C6  - pmid:39191252
UR  - <Go to ISI:>//WOS:001364262100001
DO  - DOI:10.1016/j.devcel.2024.08.001
UR  - https://bib-pubdb1.desy.de/record/614227
ER  -