Journal Article

PUBDB-2024-05681

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png A molecular pore spans the double membrane of the coronavirus replication organelle

Wolff, G. ; Limpens, R. W. A. L. ; Zevenhoven-Dobbe, J. C. ; Laugks, U.Extern*CSSB* ; Zheng, S. ; de Jong, A. W. M. ; Koning, R. I. ; Agard, D. A. ; Gruenewald, K.CSSB* ; Koster, A. J. ; Snijder, E. J. ; Bárcena, M. (Corresponding author)

2020
Assoc. Washington, DC

This record in other databases:      

Please use a persistent id in citations: doi:10.1126/science.abd3629  doi:10.3204/PUBDB-2024-05681

Abstract: Coronavirus genome replication is associated with virus-induced cytosolic double-membrane vesicles, which may provide a tailored microenvironment for viral RNA synthesis in the infected cell. However, it is unclear how newly synthesized genomes and messenger RNAs can travel from these sealed replication compartments to the cytosol to ensure their translation and the assembly of progeny virions. In this study, we used cellular cryo–electron microscopy to visualize a molecular pore complex that spans both membranes of the double-membrane vesicle and would allow export of RNA to the cytosol. A hexameric assembly of a large viral transmembrane protein was found to form the core of the crown-shaped complex. This coronavirus-specific structure likely plays a key role in coronavirus replication and thus constitutes a potential drug target.

Note: ISSN 1095-9203 not unique: **3 hits**.

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Contributing Institute(s):

1. CSSB - Leibniz-Institut für Experimentelle Virologie (LIV) - Kay Grünewald (CSSB-LIV-KG)
2. CSSB-CF-CRYO (CSSB-CF-CRYO)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)
2. DFG project 390874280 - EXC 2155: RESIST - Resolving Infection Susceptibility (390874280) (390874280)
3. SCORE - Swift COronavirus therapeutics REsponse (101003627) (101003627)

Experiment(s):

1. No specific instrument

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Database coverage:
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