Journal Article

PUBDB-2023-03810

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Bacterial c-di-GMP has a key role in establishing host–microbe symbiosis

Obeng, N. ; Czerwinski, A. ; Schütz, D. ; Michels, J. ; Leipert, J. ; Bansept, F. ; Garcia Garcia, M.DESY* ; Schultheiß1†, T. ; Kemlein, M. ; Fuß, J. ; Traulsen, A. ; Sondermann, H.CSSB*DESY* ; Tholey, A. ; Schulenburg, H. (Corresponding author)

2023
Nature Publishing Group London

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Please use a persistent id in citations: doi:10.1038/s41564-023-01468-x  doi:10.3204/PUBDB-2023-03810

Abstract: Most microbes evolve faster than their hosts and should therefore driveevolution of host–microbe interactions. However, relatively little is knownabout the characteristics that define the adaptive path of microbes to hostassociation. Here we identified microbial traits that mediate adaptation tohosts by experimentally evolving the free-living bacterium Pseudomonaslurida with the nematode Caenorhabditis elegans as its host. After tenpassages, we repeatedly observed the evolution of beneficial host-specialistbacteria, with improved persistence in the nematode being associatedwith increased biofilm formation. Whole-genome sequencing revealedmutations that uniformly upregulate the bacterial second messenger,cyclic diguanylate (c-di-GMP). We subsequently generated mutants withupregulated c-di-GMP in different Pseudomonas strains and species, whichconsistently increased host association. Comparison of pseudomonadgenomes from various environments revealed that c-di-GMP underliesadaptation to a variety of hosts, from plants to humans. This study indicatesthat c-di-GMP is fundamental for establishing host association.

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Contributing Institute(s):

1. Strukturelle Mikrobiologie CSSB (CSSB-DESY-HS)
2. Functional Genetics of Infektion Proz. (FS-FGIP)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2023

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DEAL Nature ; Essential Science Indicators ; IF >= 25 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

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