TY  - JOUR
AU  - Obeng, Nancy
AU  - Czerwinski, Anna
AU  - Schütz, Daniel
AU  - Michels, Jan
AU  - Leipert, Jan
AU  - Bansept, Florence
AU  - Garcia Garcia, Maria
AU  - Schultheiß1†, Thekla
AU  - Kemlein, Melinda
AU  - Fuß, Janina
AU  - Traulsen, Arne
AU  - Sondermann, Holger
AU  - Tholey, Andreas
AU  - Schulenburg, Hinrich
TI  - Bacterial c-di-GMP has a key role in establishing host–microbe symbiosis
JO  - Nature microbiology
VL  - 8
IS  - 10
SN  - 2058-5276
CY  - London
PB  - Nature Publishing Group
M1  - PUBDB-2023-03810
SP  - 1809 - 1819
PY  - 2023
AB  - Most microbes evolve faster than their hosts and should therefore driveevolution of host–microbe interactions. However, relatively little is knownabout the characteristics that define the adaptive path of microbes to hostassociation. Here we identified microbial traits that mediate adaptation tohosts by experimentally evolving the free-living bacterium Pseudomonaslurida with the nematode Caenorhabditis elegans as its host. After tenpassages, we repeatedly observed the evolution of beneficial host-specialistbacteria, with improved persistence in the nematode being associatedwith increased biofilm formation. Whole-genome sequencing revealedmutations that uniformly upregulate the bacterial second messenger,cyclic diguanylate (c-di-GMP). We subsequently generated mutants withupregulated c-di-GMP in different Pseudomonas strains and species, whichconsistently increased host association. Comparison of pseudomonadgenomes from various environments revealed that c-di-GMP underliesadaptation to a variety of hosts, from plants to humans. This study indicatesthat c-di-GMP is fundamental for establishing host association.
LB  - PUB:(DE-HGF)16
C6  - pmid:37653009
UR  - <Go to ISI:>//WOS:001085175700002
DO  - DOI:10.1038/s41564-023-01468-x
UR  - https://bib-pubdb1.desy.de/record/586169
ER  -