Journal Article

PUBDB-2023-03387

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography

Banari, A.CSSB* ; Samanta, A. K.CFEL*DESY* ; Munke, A. C.CFEL*DESY* ; Laugks, T.CSSB* ; Bajt, S.CFEL*DESY* ; Chapman, H. N.CFEL*DESY* ; Gruenewald, K.DESY* ; Marlovits, T.CSSB* ; Küpper, J.CFEL*DESY* ; Maia, F.Extern* ; Oberthür, D.CFEL*DESY* ; Seuring, C. (Corresponding author)CSSB*

2025
Nature Publishing Group London [u.a.]

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Please use a persistent id in citations: doi:10.1038/s41592-025-02659-6

Abstract: Structural biology offers a window into the functionality of molecularmachines in health and disease. A fundamental challenge lies in capturingboth the high-resolution structural details and dynamic changes that areessential for function. The high-resolution methods of X-ray crystallographyand electron cryo-microscopy (cryo-EM) are mainly used to studyensembles of static conformations but can also capture crucial dynamictransition states. Here, we review the latest strategies and advancementsin time-resolved structural biology with a focus on capturing dynamicchanges. We describe recent technology developments for time-resolvedsample preparation and delivery in the cryo-EM and X-ray fields and explorehow these technologies could mutually benefit each other to advance ourunderstanding of biology at the molecular and atomic scales.

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Contributing Institute(s):

1. FS-Arbeitsgruppe (FS-ML)
2. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
3. CFEL-CMI (FS-CFEL-CMI)
4. CSSB-UKE-TM (CSSB-UKE-TM)
5. CSSB - Leibniz-Institut für Experimentelle Virologie (LIV) - Kay Grünewald (CSSB-LIV-KG)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. AIM, DFG project G:(GEPRIS)390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)

Experiment(s):

1. Experiments at CFEL
2. (Advanced Light and Fluorescence Microscopy Facility)

Appears in the scientific report 2025

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Nature ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 40 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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