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001     585114
005     20250903143016.0
024 7 _ |a 10.1038/s41592-025-02659-6
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100 1 _ |a Banari, Amir
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245 _ _ |a Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography
260 _ _ |a London [u.a.]
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520 _ _ |a Structural biology offers a window into the functionality of molecularmachines in health and disease. A fundamental challenge lies in capturingboth the high-resolution structural details and dynamic changes that areessential for function. The high-resolution methods of X-ray crystallographyand electron cryo-microscopy (cryo-EM) are mainly used to studyensembles of static conformations but can also capture crucial dynamictransition states. Here, we review the latest strategies and advancementsin time-resolved structural biology with a focus on capturing dynamicchanges. We describe recent technology developments for time-resolvedsample preparation and delivery in the cryo-EM and X-ray fields and explorehow these technologies could mutually benefit each other to advance ourunderstanding of biology at the molecular and atomic scales.
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536 _ _ |a AIM, DFG project G:(GEPRIS)390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994)
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700 1 _ |a Samanta, Amit Kumar
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700 1 _ |a Munke, Anna Carina
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700 1 _ |a Laugks, Tim
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700 1 _ |a Bajt, Sasa
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700 1 _ |a Chapman, Henry N.
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700 1 _ |a Gruenewald, Kay
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700 1 _ |a Marlovits, Thomas
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700 1 _ |a Küpper, Jochen
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700 1 _ |a Maia, Filipe
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700 1 _ |a Oberthür, Dominik
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700 1 _ |a Seuring, Carolin
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773 _ _ |a 10.1038/s41592-025-02659-6
|g Vol. 22, no. 7, p. 1420 - 1435
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