TY  - JOUR
AU  - Banari, Amir
AU  - Samanta, Amit Kumar
AU  - Munke, Anna Carina
AU  - Laugks, Tim
AU  - Bajt, Sasa
AU  - Chapman, Henry N.
AU  - Gruenewald, Kay
AU  - Marlovits, Thomas
AU  - Küpper, Jochen
AU  - Maia, Filipe
AU  - Oberthür, Dominik
AU  - Seuring, Carolin
TI  - Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography
JO  - Nature methods
VL  - 22
IS  - 7
SN  - 1548-7091
CY  - London [u.a.]
PB  - Nature Publishing Group
M1  - PUBDB-2023-03387
SP  - 1420 - 1435
PY  - 2025
N1  - Waiting for fulltext 
AB  - Structural biology offers a window into the functionality of molecularmachines in health and disease. A fundamental challenge lies in capturingboth the high-resolution structural details and dynamic changes that areessential for function. The high-resolution methods of X-ray crystallographyand electron cryo-microscopy (cryo-EM) are mainly used to studyensembles of static conformations but can also capture crucial dynamictransition states. Here, we review the latest strategies and advancementsin time-resolved structural biology with a focus on capturing dynamicchanges. We describe recent technology developments for time-resolvedsample preparation and delivery in the cryo-EM and X-ray fields and explorehow these technologies could mutually benefit each other to advance ourunderstanding of biology at the molecular and atomic scales.
LB  - PUB:(DE-HGF)16
C6  - pmid:40312512
DO  - DOI:10.1038/s41592-025-02659-6
UR  - https://bib-pubdb1.desy.de/record/585114
ER  -