%0 Journal Article
%A Banari, Amir
%A Samanta, Amit Kumar
%A Munke, Anna Carina
%A Laugks, Tim
%A Bajt, Sasa
%A Chapman, Henry N.
%A Gruenewald, Kay
%A Marlovits, Thomas
%A Küpper, Jochen
%A Maia, Filipe
%A Oberthür, Dominik
%A Seuring, Carolin
%T Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography
%J Nature methods
%V 22
%N 7
%@ 1548-7091
%C London [u.a.]
%I Nature Publishing Group
%M PUBDB-2023-03387
%P 1420 - 1435
%D 2025
%Z Waiting for fulltext 
%X Structural biology offers a window into the functionality of molecularmachines in health and disease. A fundamental challenge lies in capturingboth the high-resolution structural details and dynamic changes that areessential for function. The high-resolution methods of X-ray crystallographyand electron cryo-microscopy (cryo-EM) are mainly used to studyensembles of static conformations but can also capture crucial dynamictransition states. Here, we review the latest strategies and advancementsin time-resolved structural biology with a focus on capturing dynamicchanges. We describe recent technology developments for time-resolvedsample preparation and delivery in the cryo-EM and X-ray fields and explorehow these technologies could mutually benefit each other to advance ourunderstanding of biology at the molecular and atomic scales.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:40312512
%R 10.1038/s41592-025-02659-6
%U https://bib-pubdb1.desy.de/record/585114