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@ARTICLE{Banari:585114,
author = {Banari, Amir and Samanta, Amit Kumar and Munke, Anna Carina
and Laugks, Tim and Bajt, Sasa and Chapman, Henry N. and
Gruenewald, Kay and Marlovits, Thomas and Küpper, Jochen
and Maia, Filipe and Oberthür, Dominik and Seuring,
Carolin},
title = {{A}dvancing time-resolved structural biology: latest
strategies in cryo-{EM} and {X}-ray crystallography},
journal = {Nature methods},
volume = {22},
number = {7},
issn = {1548-7091},
address = {London [u.a.]},
publisher = {Nature Publishing Group},
reportid = {PUBDB-2023-03387},
pages = {1420 - 1435},
year = {2025},
note = {Waiting for fulltext},
abstract = {Structural biology offers a window into the functionality
of molecularmachines in health and disease. A fundamental
challenge lies in capturingboth the high-resolution
structural details and dynamic changes that areessential for
function. The high-resolution methods of X-ray
crystallographyand electron cryo-microscopy (cryo-EM) are
mainly used to studyensembles of static conformations but
can also capture crucial dynamictransition states. Here, we
review the latest strategies and advancementsin
time-resolved structural biology with a focus on capturing
dynamicchanges. We describe recent technology developments
for time-resolvedsample preparation and delivery in the
cryo-EM and X-ray fields and explorehow these technologies
could mutually benefit each other to advance
ourunderstanding of biology at the molecular and atomic
scales.},
cin = {FS-ML / CFEL-I / FS-CFEL-CMI / CSSB-UKE-TM / CSSB-LIV-KG},
ddc = {610},
cid = {I:(DE-H253)FS-ML-20120731 / I:(DE-H253)CFEL-I-20161114 /
I:(DE-H253)FS-CFEL-CMI-20220405 /
I:(DE-H253)CSSB-UKE-TM-20210520 /
I:(DE-H253)CSSB-LIV-KG-20220525},
pnm = {633 - Life Sciences – Building Blocks of Life: Structure
and Function (POF4-633) / AIM, DFG project
G:(GEPRIS)390715994 - EXC 2056: CUI: Advanced Imaging of
Matter (390715994)},
pid = {G:(DE-HGF)POF4-633 / G:(GEPRIS)390715994},
experiment = {EXP:(DE-H253)CFEL-Exp-20150101 /
EXP:(DE-H253)ALFM-20250101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40312512},
doi = {10.1038/s41592-025-02659-6},
url = {https://bib-pubdb1.desy.de/record/585114},
}