Journal Article

PUBDB-2022-04937

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers

Holmes, S. ; Kirkwood, H. J. ; Bean, R. ; Giewekemeyer, K. ; Martin, A. V. ; Hadian-Jazi, M. ; Wiedorn, M. O. ; Oberthür, D. ; Marman, H. ; Adriano, L. ; Al-Qudami, N. ; Bajt, S.CFEL*XFEL.EU*DESY* ; Barák, I. ; Bari, S.DESY* ; Bielecki, J. ; Brockhauser, S. ; Coleman, M. A. ; Cruz-Mazo, F. ; Danilevski, C. ; Dörner, K. ; Gañán-Calvo, A. M. ; Graceffa, R. ; Fanghor, H. ; Heymann, M. ; Frank, M. ; Kaukher, A. ; Kim, Y. ; Kobe, B. ; Knoška, J. ; Laurus, T. ; Letrun, R. ; Maia, L. ; Messerschmidt, M. ; Metz, M. ; Michelat, T. ; Mills, G. ; Molodtsov, S.XFEL.EU* ; Monteiro, D. C. F. ; Morgan, A. J. ; Münnich, A.XFEL.EU* ; Peña Murillo, G. E.CFEL*Extern*XFEL.EU*DESY* ; Previtali, G.XFEL.EU* ; Round, A. ; Sato, T. ; Schubert, R.XFEL.EU* ; Schulz, J.XFEL.EU* ; Shelby, M. ; Seuring, C.CFEL*CSSB*XFEL.EU* ; Sellberg, J. A. ; Sikorski, M.XFEL.EU* ; Silenzi, A. ; Stern, S. ; Sztuk-Dambietz, J. ; Szuba, J. ; Trebbin, M.XFEL.EU* ; Vagovic, P. ; Ve, T. ; Weinhausen, B. ; Wrona, K. ; Xavier, P. L. ; Xu, C. ; Yefanov, O. ; Nugent, K. A. ; Chapman, H. N.CFEL*XFEL.EU*DESY* ; Mancuso, A. P. ; Barty, A.XFEL.EU*DESY* ; Abbey, B. (Corresponding author) ; Darmanin, C. (Corresponding author)

2022
Nature Publishing Group UK [London]

This record in other databases:        

Please use a persistent id in citations: doi:10.1038/s41467-022-32434-6  doi:10.3204/PUBDB-2022-04937

Abstract: The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX.

---

Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
2. Forschungsgruppe für strukturelle Dynamik (MPSD)
3. FS-Arbeitsgruppe (FS-ML)
4. Biomoleküle in Gasphase (FS-BIG)
5. CSSB-CF-CRYO (CSSB-CF-CRYO)
6. SPB/SFX (XFEL_E1_SPB/SFX)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. DFG project 194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)
3. DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)

Experiment(s):

1. XFEL Beamline SASE 1 (XFEL)

Appears in the scientific report 2022

---

Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF >= 10 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---