TY  - JOUR
AU  - Holmes, Susannah
AU  - Kirkwood, Henry J.
AU  - Bean, Richard
AU  - Giewekemeyer, Klaus
AU  - Martin, Andrew V.
AU  - Hadian-Jazi, Marjan
AU  - Wiedorn, Max O.
AU  - Oberthür, Dominik
AU  - Marman, Hugh
AU  - Adriano, Luigi
AU  - Al-Qudami, Nasser
AU  - Bajt, Saša
AU  - Barák, Imrich
AU  - Bari, Sadia
AU  - Bielecki, Johan
AU  - Brockhauser, Sandor
AU  - Coleman, Mathew A.
AU  - Cruz-Mazo, Francisco
AU  - Danilevski, Cyril
AU  - Dörner, Katerina
AU  - Gañán-Calvo, Alfonso M.
AU  - Graceffa, Rita
AU  - Fanghor, Hans
AU  - Heymann, Michael
AU  - Frank, Matthias
AU  - Kaukher, Alexander
AU  - Kim, Yoonhee
AU  - Kobe, Bostjan
AU  - Knoška, Juraj
AU  - Laurus, Torsten
AU  - Letrun, Romain
AU  - Maia, Luis
AU  - Messerschmidt, Marc
AU  - Metz, Markus
AU  - Michelat, Thomas
AU  - Mills, Grant
AU  - Molodtsov, Serguei
AU  - Monteiro, Diana C. F.
AU  - Morgan, Andrew J.
AU  - Münnich, Astrid
AU  - Peña Murillo, Gisel E.
AU  - Previtali, Gianpietro
AU  - Round, Adam
AU  - Sato, Tokushi
AU  - Schubert, Robin
AU  - Schulz, Joachim
AU  - Shelby, Megan
AU  - Seuring, Carolin
AU  - Sellberg, Jonas A.
AU  - Sikorski, Marcin
AU  - Silenzi, Alessandro
AU  - Stern, Stephan
AU  - Sztuk-Dambietz, Jola
AU  - Szuba, Janusz
AU  - Trebbin, Martin
AU  - Vagovic, Patrick
AU  - Ve, Thomas
AU  - Weinhausen, Britta
AU  - Wrona, Krzysztof
AU  - Xavier, Paul Lourdu
AU  - Xu, Chen
AU  - Yefanov, Oleksandr
AU  - Nugent, Keith A.
AU  - Chapman, Henry N.
AU  - Mancuso, Adrian P.
AU  - Barty, Anton
AU  - Abbey, Brian
AU  - Darmanin, Connie
TI  - Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers
JO  - Nature Communications
VL  - 13
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publishing Group UK
M1  - PUBDB-2022-04937
SP  - 4708
PY  - 2022
AB  - The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX.
LB  - PUB:(DE-HGF)16
C6  - pmid:35953469
UR  - <Go to ISI:>//WOS:000840107100020
DO  - DOI:10.1038/s41467-022-32434-6
UR  - https://bib-pubdb1.desy.de/record/482764
ER  -